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Position: Home > Articles > 白叶1号白化过程中叶绿体蛋白质组差异分析 Journal of Tea Science 2019 (3)

白叶1号白化过程中叶绿体蛋白质组差异分析

作  者：
李勤;程晓梅;李永迪;杨培迪;黄建安;刘仲华
关键词：
白叶1号;阶段性白化;叶绿体蛋白质组;双向电泳
摘  要：
白叶1号是一种温度敏感型白化茶树品种,叶绿体的变化是其产生阶段性白化现象的关键因素.本研究以白叶1号鲜叶叶绿体为研究对象,采用双向电泳、质谱鉴定结合生物信息学分析,研究阶段性白化过程中叶绿体蛋白的表达差异,探讨白叶1号阶段性白化现象的分子机制.结果表明,在白叶1号白化前期、白化期和复绿期叶绿体中分别识别726、748、718个蛋白质,其中差异表达的蛋白59个,质谱成功鉴定22个差异表达蛋白.生物信息学分析表明,差异表达蛋白直接或间接参与了光合作用、应激响应、核酸代谢、物质代谢和未知功能等,其中与光合作用相关的差异表达蛋白最多,占31.82%,表明阶段性白化现象可能与这些生理功能相关.通过荧光定量PCR分析发现,差异蛋白的基因表达与蛋白表达存在一定差异,这可能是由于蛋白质翻译后加工及修饰造成的.上述研究为进一步揭示白叶1号阶段性白化现象产生的分子机制奠定了理论基础.
作  者：
LI Qin;CHENG Xiaomei;LI Yongdi;YANG Peidi;HUANG Jian'an;LIU Zhonghua;Tea Key Lab of the Ministry of National Teaching of Hunan Agricultural University;National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients;Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients;Tea Research Institute, Hunan Academy of Agriculture Sciences;
单  位：
LI Qin%CHENG Xiaomei%LI Yongdi%YANG Peidi%HUANG Jian'an%LIU Zhonghua%Tea Key Lab of the Ministry of National Teaching of Hunan Agricultural University%National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients%Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients%Tea Research Institute, Hunan Academy of Agriculture Sciences
关键词：
Baiye 1;;periodic albinism;;chloroplast proteome;;2-DE
摘  要：
‘Baiye 1' is a kind of temperature sensitive tea cultivar. The change of chloroplast is the key factor for the periodic albinism of ‘Baiye 1'. To understand the mechanism of periodic albinism of ‘Baiye 1', two dimensional electrophoresis(2-DE) and mass spectrometry was adopted to separate and identify the chloroplast proteins, which were significantly changed during the three developmental periods. The results show that 726, 748 and 718 protein spots were separated at the pre-albinistic, albinistic and regreen stages, respectively. The expression levels of 59 protein spots varied markedly during the three development stages. A total of 22 protein spots were successfully identified by MS, which were involved in photosynthesis, stress response, metabolism of nucleic acid, substance metabolism and unknown function. Photosynthetic proteins were the most affected proteins, which account for31.82% in the significantly changed proteins. These results indicate that these physiological processes might play crucial roles in the periodic albinism. The gene expression profiles of the differentially expressed proteins were also verified by real-time PCR analysis. The results show that the expressions of genes and proteins were not consistent,which might be related to the protein processing and post-modification. These results provide a theoretical basis for understanding the molecular mechanism of periodic albinism in ‘Baiye 1'.

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