单 位：
中国科学院海洋研究所海洋生态与环境科学重点实验室
关键词：
织纹螺;毒性;河豚毒素;麻痹性贝毒;高效液相色谱;色谱-质谱联用
摘 要：
为了解福建沿海织纹螺毒性的动态变化,于2006年3月至9月,对福建省涵江、同安和霞浦三地的织纹螺毒性消长情况进行了跟踪监测,并对高毒性织纹螺样品中的毒素成分进行了分析。期间每周采样一次,采集的织纹螺经鉴定后,通过小鼠生物测试法分析其毒性,并选择毒性较高的织纹螺样品,应用高效液相色谱(HPLC)和高效液相色谱-质谱联用(LC-MS)技术分别对样品中的麻痹性贝毒(paralytic shellfish poison,PSP)和河豚毒素(tetrodotoxin,TTX)进行了分析。研究结果表明,三个采样点的织纹螺主要为半褶织纹螺,且均有阳性样品检出,涵江、同安和霞浦的织纹螺样品中阳性检出率分别为14%,43%和20%。采自福建三地的织纹螺样品总体毒性较低,毒性最高的样品为7月12日采自霞浦的半褶织纹螺,毒性为16.2MU.g-1组织(湿重)。对高毒性织纹螺样品的毒素分析结果表明,样品中不含麻痹性贝毒毒素,但是在样品中检测到了河豚毒素及其同系物三脱氧河豚毒素(trideoxy TTX)。样品中河豚毒素含量为4.38μg.g-1组织(湿重),依据样品中河豚毒素含量计算得到的毒性与小鼠法测试结果基本相当,说明河豚毒素及其同系物是导致织纹螺毒性的主要毒素成分。涵江的阳性样品集中出现在3月份,而同安和霞浦的阳性样品则出现了3月份和6,7月份两个高峰时段,说明织纹螺的毒性变化具有一定的季节和地域特征。因此,建议福建地区在这两个时段加强对织纹螺毒性的监测。
译 名：
Toxicity dynamics and toxin composition of Nassarius spp. from Fujian Province
作 者：
LUO Xuan1,2,YU Ren-cheng1,WANG Xiao-jie1,2,LI Jun1,ZHOU Ming-jiang1(1.Key Laboratory of Marine Ecology and Environment Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China)
关键词：
Nassarius spp.;toxicity;tetrodotoxin;paralytic shellfish poisoning toxins;high performance liquid chromato graphy;liquid chromatography-mass speetrometry
摘 要：
Consumption of gastropod Nassarius spp.caused many poisoning incidents along the coast of China.However,little information is available on the dynamics of toxicity in nassariids,which leads to the difficulty in effective prediction and prevention of the poisoning incidents.To find out the pattern of toxicity dynamics,samples of Nassarius spp.were collected from Hanjiang,Tong'an and Xiapu of Fujian Province once a week from March to September in 2006,and the toxicity of the samples was screened with the mouse bioassay method.The sample with the highest toxicity was then picked out and analyzed for paralytic shellfish poisoning toxins(PSP)and tetrodotoxin(TTX)with high performance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS),respectively.It was found that the snail samples collected from Hanjiang were mainly Nassarius semiplicatus,together with small amounts of N.reticunassa.The samples from Tong'an comprised the unidentified Nassarius sp.and small amounts of N.pullus,and the samples from Xiapu were mainly composed of N.semiplicatus and a Nassarius species unidentified.Toxic samples,which were defined as those showed positive results in mouse bioassay,were detected in all the three locations during the sampling season.There were 6 toxic samples out of the 44 samples collected from Hanjiang,and the proportion of toxic samples was 14%.In Tong'an,9 toxic samples were detected out of the 21 samples collected,and the proportion of the toxic samples was 43%.In Xiapu,5 toxic samples were detected in the 25 samples collected,and the proportion of toxic samples was 20%.For most of the toxic samples,the toxicities were lower than 4 MU·g-1,and the highest toxicity recorded was 16.2 MU ·g-1 in a sample collected from Xiapu on July 12th.For the sample with the highest toxicity,no PSP toxins were detected with HPLC.However,TTX and its analogue,trideoxy TTX,were detected in this sample with LC-MS using a SeQuant ZIC-HILIC silica column.TTX content in this sample is 4.38 μg·g-1,but the content of trideoxyTTX was not determined due to the lack of standard.Toxicity calculated based on the content of TTX(19.9 MU·g-1)is roughly in accordance with that from the mouse bioassay experiment.The toxic samples in Hanjiang were mainly found in March,but in the other two sampling sites,toxic samples were found both in March and in a period from June to July.The results indicate that TTX and its analogues are responsible for the toxicity in this batch of snail samples collected in Fujian Province,and both seasonal factors and geographical factors can affect the dynamics of snail toxicity.Based on these findings,we suggest that special attentions should be paid to the toxicity of Nassarius spp.,particularly in March,June and early July in these regions every year.
