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Position: Home > Articles > Effects of the Specificity Antagonist for Miniature Pigs Combined Anaesthetic on the Transcription of c-jun Gene mRNA in the Hippocampus of Rats Anesthetized with XFM Chinese Journal of Animal and Veterinary Sciences 2012,43 (10) 1664-1668

小型猪特异性麻醉颉颃剂对XFM麻醉下大鼠海马c-jun基因mRNA转录的影响

作者：

李小波;尹柏双;李志强;石星星;范宏刚;王洪斌

单位：

东北农业大学动物医学学院

关键词：

小型猪特异性麻醉颉颃剂;海马;c-jun;催醒机制

摘要：

本试验旨在研究小型猪特异性麻醉颉颃剂对小型猪特异性麻醉剂(XFM)麻醉下大鼠海马脑区c-jun基因mRNA转录的影响,藉以探究其催醒机制。90只成年SD大鼠被随机分成XFM麻醉组、XFM麻醉+生理盐水组、XFM麻醉+小型猪特异性麻醉颉颃剂组,每组分为5个不同采样时间点的亚组。采用实时荧光定量PCR技术检测样品中c-jun基因mRNA转录量。结果显示,XFM麻醉诱导大鼠海马脑区c-jun基因mRNA转录,各时间点海马脑区c-jun基因mRNA转录与0min比较显著升高(P<0.01),XFM麻醉大鼠腹腔注射生理盐水后各时间点海马脑区c-jun基因mRNA转录与XFM对照组间无显著差异(P>0.05),注射小型猪特异性麻醉颉颃剂后抑制了XFM诱导大鼠海马c-jun基因mRNA的转录,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果表明,小型猪特异性麻醉颉颃剂抑制了XFM麻醉诱导大鼠海马脑区c-jun基因mRNA的转录,这可能与小型猪特异性麻醉颉颃剂催醒机制相关。

译名：

Effects of the Specificity Antagonist for Miniature Pigs Combined Anaesthetic on the Transcription of c-jun Gene mRNA in the Hippocampus of Rats Anesthetized with XFM

作者：

LI Xiao-bo1,YIN Bai-shuang1,2,LI Zhi-qiang1,SHI Xing-xing1, FAN Hong-gang1,WANG Hong-bin1(1.College of Animal Medicine,Northeast Agricultural University,Harbin 150030,China; 2.Department of Animal Medicine,Jilin Agricultural Science and Technology College, Jilin 132101,China)

关键词：

the specificity antagonist for miniature pigs combined anaesthetic;hippocampus;c-jun gene;the antagonism mechanism

摘要：

To explore the antagonism mechanism of the specificity antagonist for miniature pigs combined anaesthetic,the effect of the specificity antagonist on the c-jun gene transcription in the hippocampus of rats anesthetized with XFM was studied in this experiment.Ninety adult SD rats were divided randomly into three groups: XFM group,XFM add saline group and XFM add the specificity antagonist group.Each group consists of five subgroups,and samples were collected at different time points.The transcription of c-jun gene mRNA in every sample was detected with real time PCR.The result showed that The c-jun gene mRNA contents in each time point of the XFM group were obviously increased as compared with 0 min(P<0.01).There were no significant effect of injection of saline after XFM administrated on the c-jun gene mRNA transcription(P>0.05).The level of c-jun gene mRNA transcription after the specificity antagonist administrated was significantly lower than XFM group(P<0.01 or P<0.05).These results indicated that the specificity antagonist for miniature pigs combined anaesthetic attenuates the c-jun gene mRNA transcription in the hippocampus of rats induced by XFM,this may be related to the antagonism mechanism of the specificity antagonist for miniature pigs' combined Anaesthetic.

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