中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 产肠毒素大肠埃希菌STa-K99融合蛋白重组腺病毒载体的构建及表达 动物医学进展 2013,34 (10) 30-36
Position: Home > Articles > Construction and Expression of Recombinant Adenoviral Vector with STa-K99 Fusion Protein of Enterotoxigenic Escherichia coli Progress in Veterinary Medicine 2013,34 (10) 30-36

产肠毒素大肠埃希菌STa-K99融合蛋白重组腺病毒载体的构建及表达

作  者：
邓光存;包少文;杨继辉;曾瑾;李武;刘晓明
单  位：
宁夏大学
关键词：
产肠毒素大肠埃希菌;耐热性肠毒素;黏附素;重组腺病毒载体
摘  要：
为构建产肠毒素大肠埃希菌(ETEC)STa-K99融合蛋白重组腺病毒载体Ad STa-K99,通过PCR技术分别克隆了STa及K99基因,并与腺病毒穿梭质粒连接构建了穿梭质粒pAd5 STa-K99,将pAd5STa-K99和骨架质粒(含GFP基因)分别用PacⅠ酶切线性化,利用LipofectamineTM LTX&PLUS共转染293细胞进行同源重组包装重组腺病毒,通过PCR及Western blot对重组腺病毒进行鉴定。结果显示,重组腺病毒Ad5STa-K99构建正确并表达融合蛋白,且表达的融合蛋白能够被抗体所识别,为研制由ETEC引起的新生动物腹泻重组腺病毒载体疫苗奠定了基础。
译  名：
Construction and Expression of Recombinant Adenoviral Vector with STa-K99 Fusion Protein of Enterotoxigenic Escherichia coli
作  者：
DENG Guang-cun;BAO Shao-wen;YANG Ji-hui;ZENG Jin;LI Wu;LIU Xiao-ming;Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western,Ningxia University;College of Life Science,Ningxia University;
关键词：
Enterotoxigenic Escherichia coli;;heat stable enterotoxin;;adhesion;;recombinant adenoviral vector
摘  要：
To construct recombinant adenoviral vector expressing STa-K99fusion protein of enterotoxigenic Escherichia coli,STa and K99genes were cloned and fused shuttle plasmid containing STa-K99fusion gene was constructed.Furthermore,shuttle plasmid pAd5STa-K99and backbone plasmid(containing the GFP gene)were both digested and linearized with PacⅠand co-transfected into 293cell by LipofectamineTM LTX&PLUS to generate recombinant adenovirus by homologous recombination.Finally,identification of recombinant adenovirus was conducted using PCR and Western blot.The results showed that the recombinant adenovirus Ad5STa-K99was successfully constructed and the expressed fusion protein can be recognized by anti-His antibody.Our work lays the foundation for developing novel recombinant adenoviral vector vaccine of the newborn animal diarrhea caused by ETEC.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46