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当前位置: 首页 > 文章 > 泥鳅肌肉生长抑制素基因片断的克隆及其表达 水产学报 2007,31 (2) 145-151
Position: Home > Articles > Cloning of partial sequence of myostatin of Misgurnus anguillicaudatus and its tissue expression analysis Journal of Fisheries of China 2007,31 (2) 145-151

泥鳅肌肉生长抑制素基因片断的克隆及其表达

作  者：
彭扣;陈伟伟;胡炜;王玉凤;赵浩斌
单  位：
华中师范大学生命科学学院;中国科学院水生生物研究所淡水生态与生物技术国家重点实验室
关键词：
泥鳅;肌肉生长抑制素;克隆;组织表达
摘  要：
肌肉生长抑制素(myostatin,MSTN)是动物肌肉生长发育的负调控因子。以泥鳅肌肉cDNA为模板,采用RT-PCR方法克隆了泥鳅MSTN基因的主要片段,其长度为815bp,编码271个氨基酸残基。泥鳅MSTN具有MSTN的共同特征,有蛋白酶水解位点RIRR和保守的半胱氨酸残基。RT-PCR分析表明该基因在肌肉中显著表达,在眼中也有微弱表达,而在其他所检测组织(脑、鳃、心脏、肝脏、肠、精巢、卵巢)未见表达。此结果表明泥鳅MSTN基因除对肌肉生长发育有调控作用以外,还可能在眼的发育中有一定作用。
译  名：
Cloning of partial sequence of myostatin of Misgurnus anguillicaudatus and its tissue expression analysis
作  者：
PENG Kou1,CHEN Wei-wei1,HU Wei2,WANG Yu-feng1,ZHAO Hao-bin1 (1.School of Life Sciences,Huazhong Normal University,Wuhan 430079,China;2.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430074,China)
关键词：
Misgurnus anguillicaudatus;myostatin;cloning;tissue expression
摘  要：
Myostatin(MSTN),a member of transforming growth factor(TGF)-β superfamily is a negative regulator of muscle development.The partial sequence of MSTN of loach(Misgurnus anguillicaudatus) was cloned by reverse transcription-polymerase chain reaction(RT-PCR) from muscle of loach.The segment of MSTN cDNA is 815 base pairs which encode 271 amino acid residues.The loach MSTN has the typical structure of MSTN,including a pre-domain,mature MSTN domain,conservative hydrolytic site(RIRR),and 7 conservative cysteine residues.The expression pattern of MSTN of loach was checked by RT-PCR,the results showed that the MSTN was expressed strongly in muscle and weakly in eyes;however there were not any detectable expression in other tissues such as brain, gill,heart,liver,intestine,testis,and ovary.The results showed that MSTN plays an important role in muscular development,and perhaps it has some functions in eye development.

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