作 者：
王联珠;李晓庆;顾晓慧;李烨;朱文嘉;宋春丽;殷邦忠
单 位：
中国水产科学院黄海水产研究所;安捷伦科技(中国)有限公司
关键词：
干海参;总糖;测定;外源性;苯酚-硫酸法
摘 要：
建立市场中掺糖干海参(糖干海参)的检测方法。对提取出的外源性总糖进行测定,从而判定干海参在加工过程中是否添加了糖类物质。比较提取溶剂、乙醇体积分数、提取温度、提取时间和料液比对提取条件的影响。最终确定最佳的干海参总糖提取条件为:称取1g样品,加入50mL 80%的乙醇溶液,40℃水浴振荡提取1h。利用苯酚-硫酸法进行测定。该外源性总糖的检测方法线性范围为0～80mg/L;测试液在1h以内稳定性良好;方法精密度满足实验需求,相对标准偏差在2%以内;以淡干和盐干海参为本底,样品加标回收率在82.97%～98.68%之间,相对标准偏差为1.38%～3.45%。本方法操作简单、快速、稳定性好且准确度高,可保留参体结构性多糖。应用于市售样品进行测定,可以有效甄别出掺糖干海参。
译 名：
A Method for Determination of Exogenous Total Sugar in Dried Sea Cucumber
作 者：
WANG Lian-zhu1,LI Xiao-qing1,2,GU Xiao-hui1,2,LI Ye3,ZHU Wen-jia1,SONG Chun-li1,YIN Bang-zhong1,*(1.Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;3.Agilent Technologies(China) Co.Ltd.,Qingdao 266071,China)
关键词：
dried sea cucumber;total sugar;determination;exogenous;phenol-sulfuric acid method
摘 要：
An analytical method was established for identifying exogenous sugar in dried sea cucumber.An easy and feasible method was applied to extract total sugar from dried sea cucumber,and then the total sugar was determined to discriminate if exogenous sugar was added.In the present study,the optimal concentration of extraction solvent,extraction time,temperature,and solid-liquid ratio were explored.The results showed that the optimal conditions for extracting sugar from dried sea cucumber were determined as extraction at 40 ℃ for 60 min with shaking using 80% ethanol as the extraction at a solid/solvent ratio of 1:50(g/mL).Phenol-sulfuric acid method was used to measure the extracted exogenous total sugar.The linear range of this method was 0–80 mg/L.The tested sample had good stability within 1 h and the precision of the method met the analytical requirements with RSD less than 2%.Average recoveries from spiked samples of unsalted and salted dried sea cucumber ranged from 82.97% to 98.68%,with standard deviation between 1.38% and 3.45%.This method is simple,stable and precise,and can be used to identify exogenous sugar in dried sea cucumber without affecting endogenous polysaccharides.
