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干海参线粒体的提取与纯化研究

作  者:
单懿;薛长湖;李兆杰
单  位:
中国海洋大学生物学院
关键词:
干海参;线粒体;提取;PCR
摘  要:
以干海参为原料,采用优化后的溴化十六烷基三甲基铵(CTAB)法提取其线粒体,通过A260/A280值、琼脂糖凝胶电泳和PCR检测,对所得线粒体溶液进行定量,定性分析和综合比较,为干海参的分子生物学研究提供了一套迅速、经济和可靠的技术方案。
译  名:
Study on Extraction and Purification of Mitochondrial from Dried Sea Cucumbers
作  者:
SHAN Yi,XUE Chang-hu,LI Zhao-jie (College of Life Science Ocean University of China, Qingdao 266003, China)
关键词:
dried sea cucumbers;mitochondrial;extraction;PCR
摘  要:
The mitochondria of dried sea cucumbers extracted with optimized CTAB method. The quantitative and qualitative analyses through the calculation of A260/A280. Agarose gel electrophoresis and PCR were carried out. The results showed that comparing with other methods, the traditional CTAB method would give quicker and more, economical and dependable results.

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