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当前位置: 首页 > 文章 > 低聚半乳糖糖基化法降低牛乳α_(s1)-酪蛋白抗原性和免疫原性 中国食物与营养 2011,17 (01) 50-53
Position: Home > Articles > Reduced Antigenicity and Immunogenicity of Bovine Milk α_(s1)-casein by Conjugation with Galactooligosaccharide Food and Nutrition in China 2011,17 (01) 50-53

低聚半乳糖糖基化法降低牛乳α_(s1)-酪蛋白抗原性和免疫原性

作  者：
夏薇;张帆;赖莹;杨铮;许美玉
单  位：
北京林业大学生物科学与技术学院
关键词：
αs1-酪蛋白;低聚半乳糖;糖基化;抗原性;免疫原性
摘  要：
用等电点沉淀法从牛乳中提取酪蛋白,根据αs-酪蛋白对尿素溶液溶解特性从酪蛋白中提取αs-酪蛋白,并用阴离子交换层析使αs1-酪蛋白与αs2-酪蛋白分离。用低聚半乳糖通过在一定条件下的美拉德反应对αs1-酪蛋白进行糖基化处理,通过竞争ELISA实验检测αs1-酪蛋白糖基化产物的抗原性。结果表明,该糖基化产物的抗原抗体结合常数Kd较αs1-酪蛋白上升3.7倍,即低聚半乳糖糖基化处理使αs1-酪蛋白的抗原性得到显著降低。用动物实验和非竞争ELISA实验检测αs1-酪蛋白糖基化产物的免疫原性,结果显示免疫原性降低19%,该实验证实对降低牛乳αs1-CN抗原性和免疫原性具有显著效果,为开发较理想的、切实可行的新脱敏奶粉生产技术提供了科学依据。
译  名：
Reduced Antigenicity and Immunogenicity of Bovine Milk α_(s1)-casein by Conjugation with Galactooligosaccharide
作  者：
XIA Wei,ZHANG Fan,LAI Ying,YANG Zheng,XU Mei-yu (School of Biology Science and Technology,Beijing Forestry University,Beijing 100083,China)
关键词：
αs1-casein;galactooligosaccharides;conjugation with Saccharides;antigenicity;immunogeniciity
摘  要：
Casein was purified from bovine milk by the method of isoelectric precipitation,αs-casein was isolated from casein according to the character that be dissolved in urea solution and αs1-casein was separated with αs2-casein by anion-exchange chromatography.αs1-casein was conjugated with galactooligosaccharide by maillard reaction under certain conditions.Antigenicity of the conjugates was measured by competitive ELISA assay.Results showed the Kd(dissociation constant of antigen-antibody) of the conjugates was increased to 3.7 times compared with αs1-casein and antigenicity of αs1-casein was decreased significantly by conjugation with galactooligosaccharide.Results of animal experiments and noncompetitive ELISA assay indicated that the immunogeniciity of αs1-casein was reduced 19% by combining with galactooligosaccharide.Results showed that the treatments of conjugation with saccharides had significant effect on the reducing of the antigenicity and immunogenicity of bovine milk αs1-casein and provided scientific basis for developing ideal and practice desensitization production technology of milk.

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