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当前位置: 首页 > 文章 > 葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响 食品科学 2013,34 (7) 245-249
Position: Home > Articles > Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats FOOD SCIENCE 2013,34 (7) 245-249

葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响

作  者：
李宇杰;张召锋;戴小倩;丁叶;马小陶;姜燕飞;鲍雷;李勇
单  位：
北京大学公共卫生学院营养与食品卫生学系
关键词：
葡萄籽原花青素;糖尿病视网膜病变;糖基化终末产物;炎症因子
摘  要：
目的:观察葡萄籽原花青素(GSPE)对Ⅱ型糖尿病大鼠视网膜病变的影响.方法:雄性SD大鼠高脂饲料喂养4周后,腹腔注射链脲佐菌素(STZ)造成糖尿病模型,成模后随机分为糖尿病模型组和原花青素低、中、高剂量组;以基础饲料喂养为正常对照组.原花青素低、中、高剂量组分别为125、250、500mg/(kg.d)(以体质量计),通过每天灌胃水溶液的方式进行干预,糖尿病模型组和正常对照组每天以蒸馏水灌胃.连续饲养16周后,测定其体质量、空腹血糖、血清糖基化终末产物(AGEs)、细胞间黏附分子(ICAM-1)、血管间黏附分子(VCAM-1),镜下观察视网膜的变化,免疫组化法测定视网膜中糖基化终产物受体(RAGE)的表达.结果:GSPE干预组血糖较糖尿病模型组有一定下降,但无显著性差异(P>0.05);GSPE高剂量组可以有效降低血清中AGEs水平(P<0.05);GSPE各干预组可以有效降低血清中ICAM-1水平(P<0.05);GSPE低剂量和高剂量组可以有效降低血清中VCAM-1水平(P<0.05);从组织学上,GSPE可以减轻视网膜损伤,保持视网膜各层完整;GSPE能降低RAGE在视网膜中的表达.结论:葡萄籽原花青素可以有效改善Ⅱ型糖尿病大鼠视网膜病变.
译  名：
Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats
单  位：
Department of Nutrition and Food Hygiene,School of Public Health,Peking University,Beijing 100191,China
关键词：
grape seed proanthocyanidin extract(GSPE)%diabetic retinopathy(DR)%advanced glycation endproducts(AGEs)%inflammatory factor
摘  要：
Objective: To investigate the effect of grape seed proanthocyanidin extract(GSPE) on type Ⅱ diabetic retinopathy in rats.Methods: To establish type Ⅱ diabetic rat models,male SD rats fed high fat diet for 4 weeks were injected with streptozotocin(STZ,0.01 mol/L,30 mg/(kg.d)).The diabetic rats were randomly divided into diabetes-vehicle group(DM control group),three GSPE-treated groups.Normal rats were enrolled as normal control group.The low,medium and high-dose GSPE-treated groups were administrated at 125,250 mg/(kg.d) and 500 mg/(kg.d),respectively.DM control group and normal control group were given equal volume of distilled water.After 16 weeks,body weight,fasting plasma glucose(FPG),advanced glycation endproducts(AGEs),ICAM-1 and VCAM-1 were measured.HE stain retina paraffin section was examined under a microscope.Receptor for RAGEs was detected by SP immunohistochemical method.Results: There was no difference(P > 0.05) between DM control group and GSPE-treated groups.Compared with DM control group,AGEs in GSPE high dose group were decreased(P < 0.05),ICAM-1(all GSPE-treated groups) and VCAM-1(GSPE low and high dose groups) were decreased(P < 0.05).The retina of GSPE-treated groups had fewer damaged areas and lower RAGE expression in histology.Conclusion: GSPE has a positive effect on type Ⅱ diabetic retinopathy in rats.

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