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Position: Home > Articles > The Analysis of Genetic Diversity of Microsatellite Loci in Wuchuan Black Cattle Journal of Henan Agricultural Sciences 2012,41 (12) 141-145

务川黑牛微卫星座位遗传多样性分析

作  者:
吴芸;蒋自立;田雄;马培江
单  位:
遵义师范学院生物系;务川仡佬族苗族自治县柏村镇畜牧兽医站;务川仡佬族苗族自治县饲草饲料站
关键词:
务川黑牛;微卫星;遗传多样性;遗传变异
摘  要:
以贵州地方牛优良品种务川黑牛为研究对象,选取分布于其7条染色体上的ETH10、ILSTS033、CSSM66、BM2113、ETH225、BM1818和CSRM60共7对微卫星引物进行遗传多样性分析。结果共检测到21个等位基因,每个微卫星座位等位基因数为2~5个,7个微卫星座位均未达到Hardy-Weinberg平衡。7个位点的平均等位基因数、平均有效等位基因数、平均期望杂合度、平均多态信息含量分别为3.000 0±1.154 7、2.377 3±0.585 4、0.558 4±0.101 6、0.475 9±0.125 1,说明务川黑牛具有良好的遗传多样性。为务川黑牛种质资源保存和改良提供了试验基础。
译  名:
The Analysis of Genetic Diversity of Microsatellite Loci in Wuchuan Black Cattle
作  者:
WU Yun1*,JIANG Zi-li1,TIAN Xiong2,MA Pei-jiang3 (1.Biology Department of Zunyi Normal College,Zunyi 563000,China;2.Fodder and Forage Grass Station of Wuchuan Gelos and Miao Nationality Autonomous County,Wuchuan 564300,China;3.Animal Husbandry and Veterinary Station of Bocun Town in Wuchuan Gelos and Miao Nationality Autonomous County,Wuchuan 564300,China)
关键词:
Wuchuan black cattle;microsatellite;genetic diversity;genetic variation
摘  要:
Wuchuan black cattle is a good local breed in Guizhou province.In this study,the genetic diversity of Wuchuan black cattle was analyzed by 7 selected pairs of microsatellite loci which distribute on seven different cattle chromosomes,including ETH10,ILSTS033,CSSM66,BM2113,ETH225,BM1818 and CSRM60.The results showed that 21 alleles were found,the allele numbers of each locus changes from 2 to 5 and all of these microsatellite loci didn't reached the Hardy-Weinberg equilibrium.The average number of alleles,average effective number ofalleles,average expected heterozygosity and average polymorphism information content were3.000 0±1.154 7,2.377 3±0.585 4,0.558 4±0.101 6 and 0.475 9±0.125 1,respectively.All the data represent us a good genetic diversity of Wuchuan black cattle.To provide an experimental basis for germplasm consenation and improvement of Wuchuan black cattle.

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