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Position: Home > Articles > Determination of Sulfadiazine Illegally Added in the Enrofloxacin Injection by HPLC-PDA Chinese Journal of Veterinary Drug 2013,47 (11) 40-42

恩诺沙星注射液中非法添加磺胺嘧啶HPLC-PDA检查法的建立

作  者:
郭桂芳;杨星;董玲玲;徐嫄;郝利华
单  位:
中国兽医药品监察所
关键词:
磺胺嘧啶;恩诺沙星注射液;HPLC-PDA
摘  要:
对恩诺沙星注射液中非法添加磺胺嘧啶的检测方法进行了研究。通过比较受试样品中未知峰与对照品色谱图保留时间的一致性和光谱指数图的匹配,建立了HPLC-PDA测定法,并进行相应的方法学研究。结果显示,磺胺嘧啶回收率为99.5%,RSD=0.4%;线性方程为y=873577x+98154,R2=1;检测限为5μg/mL,表明该检测方法准确可靠,可用于恩诺沙星注射液中非法添加磺胺嘧啶的检测。
译  名:
Determination of Sulfadiazine Illegally Added in the Enrofloxacin Injection by HPLC-PDA
作  者:
GUO Gui-fang;YANG Xing;DONG Ling-ling;XU Yuan;HAO Li-hua;China Institute of Veterinary Drug Control;
关键词:
sulfadiazine;;enrofloxacin injection;;HPLC-PDA
摘  要:
To identify a method for the test of sulfadiazine illegally added in the enrofloxacin injection,and the merchant sample was researched. HPLC-PDA method was established for the determination of the preliminary screening,and sulfadiazine was last identified by comparation the retention time and the spectral graph matching of the reference chromatogram and spectrum. The average recoveries of sulfadiazine sample was 99. 5%; linear equation: y = 873577x + 98154,correlation coefficient R2= 1,the limit of was 5 μg / mL. The method was accurate and reliable,and can be used in the test of sulfadiazine mixed in the enrofloxacin injection.

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