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Position: Home > Articles > Optimization of Seed Culture Medium and Conditions for L-threonine Fermentation Hubei Agricultural Sciences 2011,50 (13) 2730-2733

L-苏氨酸发酵种子培养基及培养条件的优化

作  者:
刘柯柯;张力;韩向敏;张君胜;杨小志
单  位:
甘肃农业大学动物科技学院;江苏畜牧兽医职业技术学院
关键词:
L-苏氨酸;发酵;种子培养基;培养条件;优化
摘  要:
采用正交试验设计对菌株WS4006-7E-NUT-21 L-苏氨酸发酵的种子培养基进行优化,同时对影响种子生长的条件如种子培养时间、种子液初始pH值、种子装液量等进行了研究,最终确定了L-苏氨酸发酵的最优种子培养条件。确定了种子培养基的最佳组成为葡萄糖30 g/L、硫酸铵3 g/L、玉米浆40 g/L、酵母膏5 g/L;种子的最佳培养条件为250 mL的三角瓶中装液量25 mL,种子培养基的初始pH值7.0,培养时间16 h,接种量10%。
译  名:
Optimization of Seed Culture Medium and Conditions for L-threonine Fermentation
作  者:
LIU Ke-ke1,ZHANG Li2,HAN Xiang-min1,ZHANG Jun-sheng2,YANG Xiao-zhi2(1.Faculty of Animal Science & Technology,Gansu Agricultural University,Lanzhou 730070,China; 2.Jiangsu Vocational College of Animal Husbandry and Veterinary,Taizhou 225300,Jiangsu,China)
关键词:
L-threonine;fermentation;seed culture medium;culture conditions;optimization
摘  要:
The seed culture medium of L-threonine producing strain WS4006-7E-NUT-21 was optimized by orthogonal design.Meanwhile,the factors that might influence the growth of seeds such as cultivation time,pH,and culture volume were studied;and the optimum cultivation condition was obtained.The optimal medium composition was 30 g/L glucose,3 g/L ammonium sulfate,40 g/L corn steep and 5 g/L yeast extract.While the best seed cultivation conditions were cultivating 25 mL liquid seed in 250 mL of flask,originate pH 7.0,cultivation time 16 h and with an inoculation amount of 10%.

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