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当前位置: 首页 > 文章 > L-苏氨酸产生菌的选育 安徽农业科学 2009,37 (22) 10417-10419
Position: Home > Articles > Breeding of Producing Strain of L-Threonine Journal of Anhui Agricultural Sciences 2009,37 (22) 10417-10419

L-苏氨酸产生菌的选育

作  者：
解晓鹏;张力;杨晓志;张君胜;邵喜霞
单  位：
江苏畜牧兽医职业技术学院;甘肃农业大学
关键词：
L-苏氨酸;谷氨酸棒状杆菌;紫外线;硫酸二乙酯;诱变
摘  要：
[目的]为苏氨酸基因工程菌的构建和苏氨酸的工业化生产奠定基础。[方法]以谷氨酸棒杆菌为出发菌株,采用紫外线(UV)诱变和硫酸二乙酯(DES)复合诱变进行诱变处理并筛选出AHV抗性突变株。[结果]用细菌选择性平板选出产酸菌株266株,用纸层析法从中选出产苏氨酸菌株3株,其产苏氨酸量分别为0.18、0.12和0.08 g/L。在AHV浓度为0、1.5和3.0 g/L的平板上,菌株X1的菌落多且大;在AHV浓度为4.5 g/L的平板上,其菌落少且小;在AHV浓度为6.0和7.5 g/L的平板上仅有几个小菌落。传代试验表明只有菌株UV-3能稳定产酸,产量比出发菌株高55.6%。在AHV浓度为4.5、6.0和7.5 g/L的平板上,菌株UV-3的菌落多且大;在AHV浓度为9.0 g/L的平板上,其菌落少且小;在AHV浓度为10.5 g/L的平板上仅有几个小菌落。[结论]该试验成功选育出了一株稳定、高产并具有AHV抗性的L-苏氨酸产生菌。
译  名：
Breeding of Producing Strain of L-Threonine
作  者：
XIE Xiao-peng et al(Gansu Agricultural University,Lanzhou,Gansu 730070)
关键词：
L-Threonine;Corynebacterium glutamicum;UV;Diethyl sulfate;Mutagenesis
摘  要：
[Objective] The research aimed to lay a foundation for the construction of threonine-gene engineering bacteria and the industrial production of threonine.[Method] With Corynebacterium glutamicum as original strains,they were treated by UV mutagenesis and combined mutagenesis of UV and diethyl sulfate and their mutants with resistance to AHV were screened out.[Result] Two hundred and sixty-six producing strains of acid were selected out by bacterial-selective plate,then 3 producing strains of threonine were selected out from them by paper chromatography and their threonine outputs were 0.18,0.12 and 0.08 g/L resp.The colonies of strain X1 were many and big on the plates with AHV concn.of 0,1.5 and 3.0 g/L,they were few and small on the plates with AHV concn.of 4.5 g/L and there were only a few small colonies on the plates with AHV concn.of 6.0 and 7.5 g/L.The passage experiment showed that only the strain UV-3 could produce amino acid stably,its output was 55.6% higher than that of original strain.The colonies of strain UV-3 were many and big on the plates with AHV concn.of 4.5,6.0 and 7.5 g/L,they were few and small on the plates with AHV concn.of 9.0 g/L and there were only a few small colonies on the plates with AHV concn.of 10.5 g/L.[Conclusion] A stable and high-yield strain with resistance to AHV,which could produce L-threonine,were bred out successfully in the experiment.

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