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Position: Home > Articles > A Strain Producing High Yield of L-threonine Induced by Nitrosoguanidine Guizhou Agricultural Sciences 2010,38 (4) 107-109

亚硝基胍诱变选育L-苏氨酸高产菌的研究

作  者:
王扬;张力;李伟星;张君胜;杨晓志;张铎
单  位:
江苏畜牧兽医职业技术学院;甘肃农业大学;甘肃省科学生物研究院
关键词:
谷氨酸棒状杆菌;L-苏氨酸;亚硝基胍
摘  要:
为筛选出L-苏氨酸的高产菌,以谷氨酸棒状杆菌(Corynebacterium glu anicum)为出发菌,进行亚硝基胍(NTG)诱变处理选育试验。结果表明:筛选出蛋氨酸+赖氨酸+异亮氨酸缺陷型菌株Y-1(Met-+Lys-+Iso-),经摇瓶发酵72 h后,L-苏氨酸积累可达4.14 g/L。
译  名:
A Strain Producing High Yield of L-threonine Induced by Nitrosoguanidine
作  者:
WANG Yang1,ZHANG Li2*,LI Wei-xing1,ZHANG Jun-sheng2,YANG Xiao-zhi2,ZHANG Duo3(1.Gansu Agricultural University,Lanzhou,Gansu 730070;2.Jiangsu Vocational College of Animal Husbandry and Veterinary,Taizhou,Jiangsu 225300;3.Gansu Academy of Biological Sciences,Lanzhou,Gansu 730070,China)
关键词:
Corynebacterium glu anicum;L-threonine;nitrosoguanidine
摘  要:
Corynebacterium glu anicum was treated by nitrosoguanidine to breed strains producing high yield of L-threonine.The results showed that Y-1,a strain with Met-+Lys-+Iso-,was bred and its L-threonine accumulation amount was up to 4.14g/L after 72h shake-flask fermentation culture

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