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Position: Home > Articles > Study on Production Process Parameters of Single Cell Protein Fermented by Aspergillus niger and Candida utilis Chinese Agricultural Science Bulletin 2015,31 (11) 50-53

黑曲霉和产朊假丝酵母联合发酵生产单细胞蛋白工艺参数的研究

作  者:
郝怀志;白滨;董俊;丁文姣
单  位:
甘肃省农业科学院畜草与绿色农业研究所
关键词:
黑曲霉ZM-8;产朊假丝酵母SJ2;混菌固态发酵;单细胞蛋白
摘  要:
为利用黑曲霉和产朊假丝酵母联合发酵玉米秸秆生产单细胞蛋白,笔者利用经航天诱变筛选的高产纤维素酶活菌株黑曲霉ZM-8和产朊假丝酵母SJ2,采用混菌固态发酵方式,研究产朊假丝酵母SJ2接种量、接种时间和联合发酵时间等因素在玉米秸秆为主要原料的培养基中对发酵产物中单细胞蛋白(SCP)含量的影响。结果表明最优发酵条件为先接入1:30黑曲霉,24 h后接种5%的产朊假丝酵母,黑曲霉和产朊假丝酵母联合发酵36 h。在最优发酵工艺条件下,发酵产物中SCP的含量达到了25.44%。
译  名:
Study on Production Process Parameters of Single Cell Protein Fermented by Aspergillus niger and Candida utilis
作  者:
Hao Huaizhi;Bai Bin;Dong Jun;Ding Wenjiao;Animal Pasture and Green Agricultural Institute, Gansu Academy of Agricultural Sciences;Institute of Agricultural Quality Standards and Testing Technology, Gansu Academy of Agricultural Sciences;
关键词:
Aspergillus niger ZM-8;;Candida utilis SJ2;;mixed solid state fermentation;;single cell protein
摘  要:
To produce single cell protein, corn straw was fermented by Aspergillus niger and Candida utilis.Activity strains Aspergillus niger ZM-8 and Candida utilis SJ2 which had high yield of cellulase were screenedby space mutation. Single cell protein was produced by fermentation of corn straw using mixed solid statefermentation. Effects of inoculation quantity and vaccination time of Candida utilis SJ2, combined fermentationtime and other factors influencing yield of SCP were studied. The results showed that inoculating Aspergillus niger ZM-8 on the rate of 1:30 first, then inoculating 5% Candida utilis SJ2 after 24 hours, and fermenting for36 hours was the optimal fermentation condition. And the content of SCP in the product reached 25.44% underthe optimal condition.

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