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当前位置: 首页 > 文章 > 航天诱变黑曲霉ZM-8发酵玉米秸秆粉产β-葡萄糖苷酶的培养基组分优化 食品工业科技 2012,33 (6) 236-239+243
Position: Home > Articles > Optimization on the culture medium components of space-flight mutation strain Aspergillus niger ZM-8 fermentation corn stalk powder for producing β-glucosidase Science and Technology of Food Industry 2012,33 (6) 236-239+243

航天诱变黑曲霉ZM-8发酵玉米秸秆粉产β-葡萄糖苷酶的培养基组分优化

作  者：
马旭光;张宗舟;霍建泰;赵国婵;柳芸
单  位：
甘肃省航天育种工程技术研究中心;天水师范学院生命科学与化学学院
关键词：
黑曲霉ZM-8;玉米秸秆粉;β-葡萄糖苷酶;培养基组分
摘  要：
以玉米秸秆粉为主要原料,在单因素实验基础上利用正交实验对航天诱变高产纤维素酶菌株黑曲霉ZM-8产β-葡萄糖苷酶的培养基组分进行了优化。研究结果表明,麸皮添加量(F=32.414>F0.01=6.01)和含氮量(F=13.716>F0.01=6.01)对该菌株产酶的影响达到极显著水平,含水量达到了显著水平(F=4.251>F0.05=3.55)。该菌株产酶的最佳培养基组分为:麸皮添加量为35%,氮源为1.2%的(NH4)3PO4,含水量为250%,接种量为1∶18。在此条件下,β-葡萄糖苷酶活为42.49U/mL。
译  名：
Optimization on the culture medium components of space-flight mutation strain Aspergillus niger ZM-8 fermentation corn stalk powder for producing β-glucosidase
作  者：
MA Xu - guang1,ZHANG Zong - zhou1,HUO Jian - tai2,ZHAO Guo - chan1,LIU Yun1 (1. College of Life Science and Chemical,Tianshui Normal University,Tianshui 741001,China; 2. The Space Breeding Engineering Technology Research Center in Gansu Province,Tianshui 741030,China)
关键词：
Aspergillus niger ZM - 8;corn stalk powder;β - glucosidase;culture medium components
摘  要：
Taking corn stalk powder as the main raw material,the medium components of the aerospace mutant Aspergillus niger ZM - 8 with high cellulase activity for producing β - glucosidase by the orthogonal experiment was optimized based on the single factor experiment. The results showed that the effects of bran content(F=32. 414>F0.01=6. 01)and nitrogen content(F=13. 716>F0.01=6. 01)of the strain producing β - glucosidase were extremely significant level,the effect of water content was significant level(F=4. 251>F0.05=3. 55). The optimization medium components of the strain producing β - glucosidase was as follows:the bran content was 35%,the nitrogen source was 1. 2% of (NH4)3PO4,the water content was 250% and the inoculation amount was 1∶ 18. Under these conditions,the β - glucosidase activity reached 42. 49U/ mL.

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