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当前位置: 首页 > 文章 > 新疆牛巴贝斯虫MSA-2c可溶性融合蛋白的高效表达 新疆农业科学 2009,46 (4) 835-838
Position: Home > Articles > High-level Expression of Dissoluble MSA-2c Fusion Protein of Babesia Bovis in Xinjiang Xinjiang Agricultural Sciences 2009,46 (4) 835-838

新疆牛巴贝斯虫MSA-2c可溶性融合蛋白的高效表达

作  者：
简子健;沈炯玉;马素贞;苗中秋;孙其喆;吕伟
单  位：
新疆农业大学动物医学学院
关键词：
牛巴贝斯虫;MSA-2C基因;高效表达;纯化
摘  要：
为了提高新疆牛巴贝斯虫MSA-2c融合蛋白在大肠杆菌中的表达量,研究了不同表达条件对蛋白表达量的影响,包括温度、诱导时间以及诱导剂(IPTG)浓度等。结果表明,大肠杆菌BL21(DE3)在37℃培养约2.5 h后,添加终浓度为1 mmol/L的IPTG,在27℃振荡培养8 h时,GST-Tamsl融合蛋白表达量最高,达到3.2mg/mL。用GST-Sepharose 4B亲和层析柱对CST-MSA-2c融合蛋白进行纯化,SDS-PACE检测表明GST-Tamsl融合蛋白大小约为55 KD,与预期的分子量大小一致。MSA-2融合蛋白的优化表达为牛巴贝斯虫病的分子免疫学诊断和亚单位疫苗的研制奠定了基础。
译  名：
High-level Expression of Dissoluble MSA-2c Fusion Protein of Babesia Bovis in Xinjiang
作  者：
JIAN Zi-jian,SHEN Jiong-yu,MA Su-zhen,MIAO Zhong-qiu,SUN Qi-zhe,LU Wei (College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,China)
关键词：
Babesia bovis;;MSA-2c;;high-level expression;;purification
摘  要：
In order to improve the expressing level of MSA - 2c protein in Escherichia coli,the effects of various expressing conditions were studied,including culture temperature,inducing time and the concentration of inductor IPTG etc.The results were shown the expression of GST - MSA - 2c fusion protein reached the highest level(3.2mg/mL) in bacterial strain BL21(DE3) after pre - culture for 2.5h when 1mmol/L IPTG was added by final concentration and cultured continually for 8 h at 27℃.The SDS - PAGE analysis was shown the molecular mass of GST - MSA - 2c fusion protein purified by GST - Sepharose 4B affinity chromatography was about 55KD,conforming to the expected molecular mass of GST - MSA - 2c fusion protein.The platform was established by the optimized expression conditions of MSA - 2c for the future research on the molecularly immunological diagnosis of Babesia bovis and for the development of subunit vaccine against this disease.

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