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当前位置: 首页 > 文章 > 响应面法优化Bacillus subtilis XH-13_UV3-8产葡萄糖-1-磷酸的发酵条件 食品工业科技 2011 (8) 176-179+184
Position: Home > Articles > Optimization of fermentation condition of Glucose-1-phosphate production by Bacillus subtilis XH-13_UV3-8 with response surface methodology Science and Technology of Food Industry 2011 (8) 176-179+184

响应面法优化Bacillus subtilis XH-13_UV3-8产葡萄糖-1-磷酸的发酵条件

作  者：
史军花;许向华;赵国罡;郑鸿雁
单  位：
吉林农业大学食品科学与工程学院;吉林大学农业与生物工程学院
关键词：
响应面分析法;枯草芽孢杆菌;Plackett-Burman法;葡萄糖-1-磷酸;优化
摘  要：
应用响应面法对Bacillus subtilis XH-13_UV3-8生产葡萄糖-1-磷酸的发酵条件进行了优化。首先采用Plackett-Burman设计法对12个相关影响因素的效应进行评价,筛选出了有显著正效应的酵母浸粉浓度和K2HPO4·3H2O浓度和有显著负效应的pH等3个因素;接着用最陡爬坡路径逼近最大响应区域;最后通过Box-Behnken设计法,利用Design-Expert软件进行二次回归分析,得到主要因素的最佳条件为:酵母浸粉0.8%、K2HPO4·3H2O0.7%和pH6.6,在此优化培养条件下,发酵液中葡萄糖-1-磷酸浓度从6.85mg/mL提高到8.56mg/mL。
译  名：
Optimization of fermentation condition of Glucose-1-phosphate production by Bacillus subtilis XH-13_UV3-8 with response surface methodology
作  者：
SHI Jun-hua~1,XU Xiang-hua~1,ZHAO Guo-gang~2,ZHENG Hong-yan~1,*(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;2.School of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China)
关键词：
response surface methodology;Bacillus subtilis;Plackett-Burman design;G-1-P;optimization
摘  要：
Response surface methodology was used to optimize the fermentation condition of glucose-1-phosphate(G-1-P)production by Bacillus subtilis XH-13_UV3-8.Firstly,a Plackett-Burman design was used to evaluate the influence of 12 related factors.Yeast extract powder and K2HPO4·3H2O influenced G-1-P production positively while pH did negatively.The path of steepest ascent was used to approach the optimal region of the fermentation condition subsequently.Box-Behnken design was used to optimize the three critical factors mentioned above,the optimum levels and the relationships among these factors were found out by quadratic regression model equation with Design-Expert statistic methods,the optimal levels of the variables were determined as follows:yeast extract powder 0.8%,K2HPO4·3H2O 0.7% and pH 6.6.The optimized condition allowed the G-1-P production to increased from 6.85mg/mL to 8.56mg/mL.

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