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当前位置: 首页 > 文章 > 辣白菜中乳酸菌的分离鉴定 食品科学 2015,36 (11) 130-133
Position: Home > Articles > Isolation and Identification of Lactic Acid Bacteria from Kimchi FOOD SCIENCE 2015,36 (11) 130-133

辣白菜中乳酸菌的分离鉴定

作  者：
孟令帅;张颖;邹婷婷;乌日娜;岳喜庆;武俊瑞
单  位：
沈阳农业大学食品学院
关键词：
辣白菜;乳酸菌;16S r DNA;同源性分析
摘  要：
从沈阳市7个区25份朝鲜族家庭制作的传统发酵辣白菜中分离出81株乳酸菌疑似菌株,初步鉴定34株为杆菌,47株为球菌。进一步采用16S r DNA序列分析对81株菌进行分子鉴定,通过序列分析进行属种鉴定。结果表明:81株菌均为乳酸菌,分别来自2个属6个种,45株为屎肠球菌,25株为植物乳杆菌,4株为干酪乳杆菌,3株为戊糖乳杆菌,2株为短乳杆菌,2株为坚强肠球菌。研究结果为我国东北辣白菜中乳酸菌作进一步研究奠定了基础。
译  名：
Isolation and Identification of Lactic Acid Bacteria from Kimchi
作  者：
MENG Lingshuai;ZHANG Ying;ZOU Tingting;WU Rina;YUE Xiqing;WU Junrui;College of Food Science,Shenyang Agricultural University;State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University;
关键词：
kimchi;;Lactobacillus;;16S r DNA;;homology analysis
摘  要：
Lactic acid bacteria were isolated and identified from 25 Korean traditional fermented kimchi samples from 7 districts of Shenyang.Totally 81 strains suspected of being lactic acid bacteria were isolated successfully,and 34 of the isolated strains were identified as bacilli and 47 were identified as cocci.These 81 strains were further identified by 16 S r DNA sequence analysis.The results showed that all these 81 strains were confirmed as lactic acid bacteria from 2 genus and 6 species,including 45 strains of Enterococcus faecium,25 strains of Lactobacillus plantarum,4 strains of Lactobacillus casei,3 strains of Lactobacillus pentosus,2 strains of Lactobacillus brevis,and 2 strains of Enterococcus durans.These results can provide a basis for further studies on lactic acid bacteria from kimchi in northeast China.

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