当前位置: 首页 > 文章 > 高效液相色谱法测定芝麻油中木酚素的含量 湖北农业科学 2011,50 (4) 183-186
Position: Home > Articles > HPLC Methods of Analysis for Lignans in Sesame Oil Hubei Agricultural Sciences 2011,50 (4) 183-186

高效液相色谱法测定芝麻油中木酚素的含量

作  者:
李丹丹;曾晓雄
单  位:
南京农业大学食品科技学院
关键词:
芝麻油;芝麻酚;芝麻素;芝麻林素;高效液相色谱;测定
摘  要:
本文以芝麻油为原料,通过Kieselgel 60预处理得到木酚素混合物,利用Agilent C18分离柱,以无水甲醇-水(70∶30,V/V)为流动相,建立了芝麻油中芝麻酚、芝麻素和芝麻林素3种木酚素同时定量分析的HPLC方法。通过样品分析、重复性实验和回收率实验,分析结果表明该法用于分离芝麻油中各木酚素分离效果良好,峰形尖锐,基线平稳,可在10 min内完成,线性范围5~200μg/mL,方法精密度及回收率均较好,定量结果准确可靠,是一种简便、稳定、可靠的木酚素检测方法。同时利用所建立的方法对市售20种芝麻油和3种芝麻色拉油中木酚素进行了分析。
译  名:
HPLC Methods of Analysis for Lignans in Sesame Oil
作  者:
LI Dan-dan,ZENG Xiao-xiong(College of Food Science and Technology,Nanjing agricultural university,Nanjing 210095,China)
关键词:
sesame;sesamol;sesamin;sesamolin;HPLC;determination
摘  要:
A quantitative HPLC method for sesamol,sesamin and sesamolin in sesame oil samples was established in this paper.The sample of sesame oil was pretreated by chromatographic column packed with Kieselgel 60 and separated in the Agilent C18 column with the mobile phase of CH4O-H2O(70∶30,V/V).The HPLC analysis cost about 10 min,and the linearity range was between 5~200 μg/mL.The results showed that this method was brief,stable,and reliable for its separation shape acuity and base steadiness.The sesamol of 20 brands of sesame oil samples and 3 brands of sesame salad oil samples bought in local market were analyzed by this method.

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