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Position: Home > Articles > Separation of 15-α-OH Neoline from Aconitum carmichaeli By High-Speed Countercurrent Chromatography Journal of Jilin Agricultural University 2004,26 (1) 60-61+68

高速逆流色谱法分离附子中的15-a-羟基新乌碱

作  者:
洪波;司云珊;赵宏峰;徐雅娟
单  位:
吉林省中医中药研究院;吉林农业大学资源与环境学院
关键词:
高速逆流色谱法;分离;附子;15-α-羟基新乌碱
摘  要:
采用高速逆流色谱法对附子中的化学成分进行研究,分离得到了15 α 羟基新乌碱单体。溶剂系统为氯仿-甲醇-盐酸(0.3mol L)=4∶3∶2,上层为固定相,下层为流动相。流速1.8mL min,转速850r min。并对其结构进行了鉴定。
译  名:
Separation of 15-α-OH Neoline from Aconitum carmichaeli By High-Speed Countercurrent Chromatography
作  者:
HONG Bo~1, SI Yun-shan~2, ZHAO Hong-feng~2, XU Ya-juan~2 (1.College of Resources and Environmental Science, Jilin Agricultural University,Changchun, Jilin 130118, China; 2.Jilin Academy of Chinese Medicine, Changchun, Jilin 130021, China)
关键词:
 high speed countercurrent chromatography(HSCCC); separation; Aconitum carmichaeli; 15-a-OH Neolinei
摘  要:
Using High-Speed Countercurrent Chromatography(HSCCC),the author obtained 15-α-OH Neoline from Aconitum carmichaeli. with two-phase solvent system composed of chioroform-methanol-hydrochloric acid(4∶3∶2 in volume ratio).The mobile phase is the lower phase and operated at a flow-rate of 1.8 mL/min,while the apparatus rotated at 850 r/min. Its structure was identified.

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