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Position: Home > Articles > Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography FOOD SCIENCE 2004,25 (1) 85-88

高速逆流色谱法分离制备大豆异黄酮中的大豆苷和染料木苷

作  者:
江和源;台建祥;吕飞杰
单  位:
中国农业科学院茶叶研究所农业部茶叶化学工程重点开放实验室;中国农业科学院
关键词:
高速逆流色谱法;分离;大豆苷;染料木昔
摘  要:
采用高速逆流色谱法分离纯化大豆异黄酮中的大豆苦和染料木昔。溶剂系统为乙酸乙酯一醋酸一水,体积比为5:1:10,上相为固定相,下相为流动相,逆流色谱仪转速为800r/mm,流速为1.5ml/min。所得大豆昔、染料木昔经高效液相色谱分析测定,纯度分别达到98.2%和99.2%(面积归一法)。
译  名:
Separation of Daidzin and Genlstin from Soybean Isoflavones by High Speed Countercurrent Chromatography
作  者:
JIANG He-yuan1,TAI Jian-xiang2,LV Fei-jie2(1.Key Laboratory of Tea Chemical Engineering, Tea Research lnstitute, Chinese Academy of Agriculture Sciences,Hangzhou 310008, China;2.Chinese Academy of Agriculture Sciences,Beijing l00081, China)
关键词:
high speed countercurrent chromatography;separation; daidzin;genistin
摘  要:
Semlpreparation high speed coufltercurent chromatography(HSCCC)was used for the systematic optimization andpreparation of the two-phase solvent system to separate Daidzin and Genistin from soybean isoflavones extracted from soybeanseed.The two-phase solvent system composed of ethyl acetate-acetic acid-water(5:l:10 in volume ratio).The mobile phase is thelower phase nd operated at flow-rate of 1.5ml/min, while the apparatus rotated at 800r/min.High performance liquidchromatographic analysis of Daidzin and Genistin revealed that their purity was individually 98.2%and 99.2%.

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