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当前位置: 首页 > 文章 > 西藏小型猪线粒体DNA控制区的分子遗传学研究 畜牧兽医学报 2009,40 (6) 800-805
Position: Home > Articles > Genetic Analysis of Mitochondrial DNA D-loop Region Sequences in Tibet Mini-pigs Chinese Journal of Animal and Veterinary Sciences 2009,40 (6) 800-805

西藏小型猪线粒体DNA控制区的分子遗传学研究

作  者：
李洪涛;肖东;袁进;吴清洪;王万山;张嘉宁;顾为望
单  位：
南方医科大学实验动物中心;广州医学院第一附属医院
关键词：
西藏小型猪;线粒体DNA控制区;遗传标记;亲缘关系
摘  要：
通过扩增102头西藏小型猪以及16头巴马小型猪、17头贵州香猪的线粒体DNA控制区,测序并与国内其他猪种进行比较,研究西藏小型猪的遗传标记以及与其他国内地方猪种的亲缘关系。结果显示,西藏小型猪线粒体DNA D-loop区分3个区域。串联重复序列区处于中间位置,包含有15~29个10 bp的重复片段,分为A、B 2种类型。3′端340 bp,与国内其他猪种的序列相同,比较保守;D-loop 5′端704 bp,共有22个变异位点。由22个变异位点中归纳出25个单倍型,其中有2种主要的单倍型,分别占34.4%和36.6%。根据3个转换位点:305、500、691,将西藏小型猪分成了2组,几乎与串联重复序列所分的A、B 2组类型相对应。与西藏小型猪相比,巴马小型猪和贵州香猪D-loop 5′端变异位点较少,分别只有4种和2种单倍型,串联重复区也只有1个类型。说明西藏小型猪可能有2个母系祖先,并且与我国西南地区的猪种有较近的亲缘关系;不同的串联重复片段类型和5′端的变异位点可以联合组建西藏小型猪的遗传标记。
译  名：
Genetic Analysis of Mitochondrial DNA D-loop Region Sequences in Tibet Mini-pigs
作  者：
LI Hong-tao1,2,XIAO Dong1,YUAN Jin1,WU Qing-hong1,WANG Wan-shan1,ZHANG Jia-ning1,GU Wei-wang1*(1.Laboratory Animal Centre,Southern Medical University,Guangzhou 510515,China;2.First Affiliated Hospital of Guangzhou Medicial University,Guangzhou 510120,China)
关键词：
Tibet Mini-pig;mtDNA D-loop region;genetic marker;phylogenetic relationship
摘  要：
To analyze mitochondrial DNA D-loop region sequences of Tibet Mini-pigs,and to explore their cytoplasmic DNA markers used to identify them and the phylogenetic relationship between Tibet Mini-pigs and other local pig breeds in China,mtDNA D-loop region in 102 Tibet Mini-pigs,16 Bama miniature pigs and 17 Guizhou Xiang pigs were amplified and sequenced,followed by comparing with the data of other Chinese domestic pigs.The result showed that the D-loop region of Tibet Mini-pigs was divided into three areas.The tandem repeat sequence which had 15-29 10 bp repeat motives and located in the middle of mtDNA D-loop region consisting of type A and B.340 bp sequence at 3′ end of the D-loop which was very conservative exhibited particularly high homology with the same region of other domestic pigs.704 bp sequence at 5′ end of the D-loop had 22 polymorphic sites.22 polymorphic sites deduced 25 haplotypes,among which the percentage of two major haplotypes reached 34.4% and 36.6%,respectively.Based on three transform sites(305,500,691),Tibet Mini-pigs were divided into two groups,nearly corresponding to type A and B of tandem repeat sequence.Compared with Tibet Mini-pigs,Bama miniature pigs and Guizhou Xiang pigs had four and two haplotypes,respectively,and had type A of tandem repeat sequence.It is inferred that Tibet Mini-pigs originated from two maternal ancestors and were close relative relationship with local pigs in southwest China.The repeating motif in combination with the variable sites at the 5′end of mtDNA D-loop region can be uesd as the genetic marker to identify Tibet Mini-pigs.

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