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Position: Home > Articles > Study on the difference of methylation and expression level of IGFBP-3 gene in Tibetan Mini-pig Journal of Yangzhou University(Agricultural and Life Science Edition) 2013,34 (1) 11-15

西藏小型猪IGFBP-3基因甲基化与表达差异分析

作  者:
许无恨;马强;王佳;官员;房希碧;田来明;郝林琳;于浩;张英;刘松财
单  位:
长春市农业科学院;吉林大学畜牧兽医学院
关键词:
西藏小型猪;IGFBP-3基因;甲基化;重亚硫酸盐测序PCR
摘  要:
为研究西藏小型猪类胰岛素生长因子结合蛋白3(IGFBP-3)基因甲基化及表达差异,对IGFBP-3基因的CpG岛甲基化程度、mRNA及蛋白表达水平进行分析,并以军牧一号猪为对照。结果表明:西藏小型猪肝脏组织中IGFBP-3基因CpG岛甲基化水平显著高于军牧一号猪(P<0.05);西藏小型猪不同组织中IGFBP-3mRNA表达量均显著高于军牧一号猪(P<0.05);同时肾脏中IGFBP-3蛋白含量显著高于军牧一号猪(P<0.05)。这为猪生长发育调控及促生长机制提供了分子依据。
译  名:
Study on the difference of methylation and expression level of IGFBP-3 gene in Tibetan Mini-pig
作  者:
XU Wuhen1,MA Qiang1,WANG Jia1,GUAN Yuan1,FANG Xibi1,TIAN Laiming2, HAO Linlin1,YU Hao1,ZHANG Ying1,LIU Songcai1(1.Coll of Anim Sci and Vet Med,Jilin Univ,Changchun 130062,China; 2.Changchun City Acad of Agric Sci,Changchun 130111,China)
关键词:
Tibetan Mini-pig;IGFBP-3 gene;methylation;BSP
摘  要:
In order to investigate the difference of the methylation and the expression levels of insulin-like growth factor binding protein 3(IGFBP-3) between Tibetan Mini-pig and the Junmu-1 white pig,the methaylation of CpG island,the mRNA and the protein expression level of IGFBP-3 were detected in this study.The results showed that: The methylation level of IGFBP-3 gene in the liver of Tibetan Mini-pig was significantly higher than that in the liver of Junmu-1 pig(P<0.05).The mRNA level of IGFBP-3 in the tissues of Tibetan Mini-pig was higher than that in the Junmu-1 pig generally(P<0.05),whereas,the same phenomenon of the IGFBP-3 protein expression was only found in the kidney tissue(P<0.05).This would lay certain molecular theoretical basis for the pig resource specific to the growth and development mechanisms of pigs.

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