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当前位置: 首页 > 文章 > 火棘果红色素的提取及抗氧化活性 食品科学 2013,34 (17) 116-119
Position: Home > Articles > Extraction and Antioxidant Activity of Red Pigments from Pyracantha fortuneana FOOD SCIENCE 2013,34 (17) 116-119

火棘果红色素的提取及抗氧化活性

作  者：
李鹏霄;茆广华;赵婷;邹烨;任月娜;白石琦;吴向阳;仰榴青
单  位：
江苏大学化学化工学院;江苏大学食品与生物工程学院;江苏大学药学院
关键词：
火棘果;红色素;纯化;抗氧化
摘  要：
对火棘果红色素进行提取和纯化,并进行抗氧化活性测定。采用70%乙醇(pH3,HCl)提取火棘果红色素,用pH示差法、福林-肖卡尔法及蒽酮比色法分别测定火棘果红色素粗提物(PFE)及经C18Sep-Pak柱纯化后的产物(PPFE)中花色苷、总酚和总糖含量;同时测定PPFE对·OH、O2-·、DPPH自由基的清除作用。结果表明:PFE中花色苷含量1.16mg/100g、总酚含量2.18mg/100g、总糖含量1095.34mg/100g;PPFE中花色苷含量20.61mg/100g、总酚含量48.62mg/100g,未检测出总糖含量。PPFE清除·OH、O2-·、DPPH自由基的IC50分别为1.43mg/mL、3.13mg/mL、3.43 g/mL。提示:C18Sep-Pak柱具有除糖及富集花色苷和总酚的作用,PPFE有较强的抗氧化作用。
译  名：
Extraction and Antioxidant Activity of Red Pigments from Pyracantha fortuneana
作  者：
LI Peng-xiao;MAO Guang-hua;ZHAO Ting;ZOU Ye;REN Yue-na;BAI Shi-qi;WU Xiang-yang;YANG Liu-qing;School of Pharmacy, Jiangsu University;School of Food and Biological Engineering, Jiangsu University;School of Chemistry and Chemical Engineering, Jiangsu University;
关键词：
Pyracantha fortuneana;;red pigment;;purify;;antioxidant activity
摘  要：
Red pigments were extracted with 70% acidified ethanol(pH 3, HCl) and purified by C 18 Sep-Pak column chromatography. The radical scavenging activity of the purified pigments was assessed against · OH, O 2- · and DPPH radical. The contents of anthocyanins, total phenols and total sugar in the crude extract and the purified product were determined by pH differential method, Folin-Ciocalteau method and anthrone colorimetric method, respectively. Results showed that the contents of anthocyanin, total phenols and total sugar in the crude extract were 1.16, 2.18 mg/100 g and 1095.34 mg/100 g, respectively, while those in the purified product were 20.61, 48.62 mg/100 g and 0 mg/100 g, respectively. The IC 50 values of the purified product against · OH, O 2- · and DPPH radical were 1.43, 3.13 g/mL and 3.43 g/mL, respectively. These results indicate that the C 18 Sep-Pak column chromatography could effectively remove sugar compounds and concentrate anthocyanins and total phenols in the crude extract. The purified red pigments had a relatively strong antioxidant capacity.

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