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Position: Home > Articles > Extraction Process Optimization of Proanthocyanidins from Wild Pyracantha fortuneana Fruits Food and Nutrition in China 2011,17 (05) 60-66

火棘果中原花青素提取工艺优化

作  者:
杨博;鄢又玉;钟方旭;彭友瑞
单  位:
武汉工业学院生物与制药工程学院
关键词:
火棘果;原花青素;析因实验;响应面法
摘  要:
以野生火棘果为材料,利用乙醇溶液提取原花青素,采用铁盐催化比色法测定原花青素浓度并计算得率。在单因素实验及Plackett-Burman析因实验的基础上,通过Box-Behnken实验设计和响应面分析进一步优化料液比、乙醇浓度、pH对原花青素得率的影响。结果表明,在液料比12∶1、乙醇浓度73%、pH=3.6、提取温度80℃、提取时间60min、提取2次条件下,所获得的原花青素得率较高,可达到了93.446 mg/g。
译  名:
Extraction Process Optimization of Proanthocyanidins from Wild Pyracantha fortuneana Fruits
作  者:
YANG Bo1,YAN You-yu1,2,ZHONG Fang-xu1,PENG You-rui1 (1 College of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China;2College of Life Science and Technology,Huazhong Univrersity of Science and Technology,Wuhan 430023,China)
关键词:
Pyracantha fortuneana fruit;proanthocyanidins;factorial experiment;response surface method
摘  要:
Response surface method was used to optimize the extraction process of proanthocyanidins from wild Pyracantha fortuneana fruit.On the basis of single-factor experiments and plackett-burman design successively,Box-Behnken center-united experimental design principles were used to devise the experiments and the response surfaces analysis of 3 factors,including ratio of material to liquid,ethanol concentration and pH of extracting solvent were adopted to further optimize extracting process.Results showed that when ratio of water to material is 12:1,ethanol concentration 73%,pH 3.6,extracting temperature 80 ℃,and extraction time 60min,extraction twice,the yield of proanthocyanidin will arrive 93.446 mg/g.

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