当前位置: 首页 > 文章 > 发酵大豆食品中染料木素含量的ELISA测定 食品科学 2002,23 (7) 118-120
Position: Home > Articles > ELISA Study on Genistein Content in Fermented Soybean Food FOOD SCIENCE 2002,23 (7) 118-120

发酵大豆食品中染料木素含量的ELISA测定

作  者:
吴定;路桂红
单  位:
南京经济学院食品科学与工程系
关键词:
染料木素;酶联免疫吸附测定;发酵大豆制品
摘  要:
染料木素是大豆发酵食品中的主要异黄酮成分。制备了牛血清白蛋白-染料木素免疫抗原和卵白蛋白-染料木素包被抗原,并对它们进行紫外和SDS-聚丙稀酰胺凝胶电泳鉴定。抗染料木素抗体的效价为1:1600。建立竞争ELISA测定发酵大豆制品中染料木素含量方法,染料木素测定浓度范围为6.25~150μg/L;回收率为87%~102%;亲和常数为1.62×108;与大豆甙元、芒柄花素的交叉反应率分别为9.8%、4.57%;与槲皮素、儿茶素、单宁和核黄素等结构类似物无交叉反应。同时将ELISA法与薄层层析法进行比较。
译  名:
ELISA Study on Genistein Content in Fermented Soybean Food
作  者:
Wu Ding et al.
关键词:
Genistein Enzyme -linkedimmunosorbent assay The fermented soybeanfood
摘  要:
Genistein was the main component of i soflavone in the fermented soybean f ood.The immune antigen of BSA -genistein andthe coatedantigenof OA -genistein were synthesizedr espectively,andidentified with ul traviolet absorptionspectrometryandSDS -polyacrylamidegelelectrophoresis.TheantibodyIgGofantigenisteinwa spuredwith saturatedsolutionof(NH 4 ) 2 SO 4 andthe columnchromatography ofSep hadex G -25,with1:1600titer ofanti body IgG of antigenistein.The competitive ELISAfor detecting the genistein con tent in the fermented soybean food wa s devel-oped,with6.25~150μg /Lofthedetectingconcentrationr angeofgenistein,therecoveryof87%~102%,theaffinity constant of 1.62×10 8 ,and the 9.8%and 4.57%cross reactio n ratios of antibody for daidzin and f ormononetin re-spectively,while no cross reaction for catechin,tannin,quercetin and riboflavin was observed.The ELISAmethodand thin -leaver chromatography method were also compared for the effect of assaying genistein.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊