中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Q热贝氏柯克斯体间接ELISA检测方法的建立 中国畜牧兽医 2014,41 (4) 103-109
Position: Home > Articles > Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii China Animal Husbandry & Veterinary Medicine 2014,41 (4) 103-109

Q热贝氏柯克斯体间接ELISA检测方法的建立

作  者：
武文君;贾广乐;廖娟红;梅琳;韩雪清;林祥梅;张映
单  位：
山西农业大学动物科技学院;中国检验检疫科学研究院
关键词：
Q热;贝氏柯克斯体;间接ELISA;Ⅱ相抗原
摘  要：
本研究以Q热贝氏柯克斯体弱毒株Ⅱ相全菌为包被抗原建立Q热贝氏柯克斯体间接ELISA检测方法,通过方阵滴定法对抗原包被浓度和二抗反应浓度的确定及各项反应条件的优化,确定其阴阳性临界值为0.44。用本方法与IDEXX Q热抗体检测试剂盒对393份牛临床血清进行检测,检出的血清阳性率分别为11.45%和6.10%,符合率为94.66%。结果表明,本试验建立的检测Q热贝氏柯克斯体的间接ELISA法具有较好的特异性及较强的敏感性,可初步应用于Q热贝氏柯克斯体抗体的临床检测。
译  名：
Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii
作  者：
WU Wen-jun;JIA Guang-le;LIAO Juan-hong;MEI Lin;HAN Xue-qing;LIN Xiang-mei;ZHANG Ying;College of Animal Science and Technology,Shanxi Agricultural University;Chinese Academy of Inspection and Quarantine;
关键词：
Q fever;;Coxiella burnetii;;indirect ELISA;;phaseⅡantigen
摘  要：
In this study,the whole bacteria protein of the Coxiella burnetii attenuated strain phaseⅡ was used as the antigen and then the indirect ELISA to detect Q fever Coxiella burnetii was established.Through optimizing the concentration of the coated phaseⅡantigen,the concentration of the second antibody and the reaction condition,we confirmed that the critical value of this method was 0.44.393clinical bovine sera were detected by this method and IDEXX Q fever(Coxiella burnetii) Antibody Test Kit.The results showed that the sera positive detection rates were 11.45% and 6.10%,respectively.Compared with the results of the two detection methods,the coincidence was 94.66%.The results demonstrated that sensitivity and specificity of the established indirect ELISA detection method were better and it could be used for the clinical diagnosis of the antibody of Q fever Coxiella burnetii.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46