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Position: Home > Articles > Specimen Preparation Methods of Single Embryo Chromosome of Three Kinds of Fish Journal of Anhui Agricultural Sciences 2013 (23) 320+328

3种鱼类的单个胚胎染色体标本制备方法

作  者:
王玉生;李雅娟;李佳奇;徐雯;高养春;刘博
单  位:
大连海洋大学;农业部北方海水增养殖重点实验室
关键词:
泥鳅;红鳍东方鲀;德国镜鲤;单个胚胎;染色体
摘  要:
[目的]为建立1种准确、快捷、高效的鱼类染色体制备方法。[方法]以泥鳅、红鳍东方鲀和德国镜鲤的单个胚胎作为材料,通过秋水仙素、低渗、卡诺固定等操作制备染色体标本。[结果]3种鱼类均获得大量形态清晰、长度适中、分散良好的中期分裂相。[结论]以鱼类肌肉效应期为材料可成功制备染色体标本,为鱼类染色体标本制备提供了新方法。
译  名:
Specimen Preparation Methods of Single Embryo Chromosome of Three Kinds of Fish
作  者:
WANG Yu-sheng;Key Laboratory of Mariculture & Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University;
关键词:
Misgurnus anguillicaudatus;;Takifugu rubripes;;Cyprinus carpio L.;;Single embryo;;Chromosome
摘  要:
[Objective]The aim was to establish an accurate,raid and high efficient method of preparing fish chromosome. [Method] Single embryo of Misgurnus anguillicaudatus,Takifugu rubripes and Cyprinus carpio L. was treated successively with colchicine,hypotonic solution and Carnoy fixation to obtain chromosome specimen. [Result]A considerable amount of chromosome of with clear shape,moderate length and good dispersion were obtained from three kinds of fish. [Conclusion]Chromosome could be successfully obtained from the embryo sample of the muscle effected period,which had given a new way of chromosome preparation.

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