作 者：
樊佳奇;韩雯笑;范慧敏;陈宝江;王宏;郅永伟;崔秋佳;陈赛娟
单 位：
河北农业大学动物科技学院;北京英惠尔生物技术有限公司;内蒙古大尔多兔业有限公司;衡水市枣强县畜牧站;河北农业大学山区研究所
摘 要：
本试验旨在研究酵母肽对肉兔生长性能以及脂多糖(LPS)应激肉兔血清细胞因子和肝脏抗氧化能力的影响.试验分2个阶段进行.第1阶段:选取160只健康的35日龄断奶伊拉配套系肉兔,初始体重为(0.96±0.06)kg,随机分为4组(公母各占1/2),每组8个重复,每个重复5只.对照组(CON组)和LPS组饲喂基础饲粮,酵母肽组(YP组)和酵母肽缓解组(L-YP)饲喂添加100 mg/kg酵母肽的基础饲粮.第1阶段饲养试验共进行35 d,其中预试期7 d,正试期28 d.记录体重、采食量等数据并计算第1阶段试验期间肉兔的生长性能指标.第2阶段:于第1阶段结束时,LPS组和L-YP组试验兔大腿肌肉注射200 μg/kg BW的LPS,CON组和YP组则注射等量生理盐水,在LPS或生理盐水注射4 h后采样,测定血清细胞因子含量以及肝脏抗氧化指标和抗氧化基因mRNA相对表达量.结果显示:1)饲粮中添加酵母肽显著增加了肉兔的平均日增重(P<0.05),显著降低了料重比(P<0.05),对平均日采食量、腹泻率和死亡率无显著影响(P>0.05).2)饲粮中添加酵母肽显著降低了肉兔血清中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和活性氧(ROS)含量(P<0.05);LPS刺激显著增加了肉兔血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6、IL-8、转化生长因子-β(TGF-β)和ROS含量(P<0.05);酵母肽和LPS在血清TNF-α、IL-6、IL-10和TGF-β含量上有显著的交互效应(P<0.05).在LPS刺激条件下,饲粮中添加酵母肽可以显著降低LPS应激肉兔血清中TNF-α和IL-6含量(P<0.05).3)饲粮中添加酵母肽显著提高了肉兔肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)(P<0.05),并显著降低了丙二醛(MDA)含量(P<0.05);LPS刺激显著降低了肉兔肝脏中T-AOC和GSH-Px活性(P<0.05);酵母肽和LPS对肉兔肝脏中SOD活性和SOD的mRNA相对表达量有显著的交互效应(P<0.05).在LPS刺激条件下,在LPS刺激条件下,饲粮中添加酵母肽可以显著提高LPS应激肉兔肝脏中SOD活性和SOD的mRNA相对表达量(P<0.05).综上所述,饲粮中添加100 mg/kg酵母肽可以提高肉兔的生长性能,并通过降低机体炎症水平、提高抗氧化能力有效缓解LPS诱导的肉兔免疫应激反应.
译 名：
Effects of Yeast Peptides on Growth Performance of Meat Rabbits,and Serum Cytokines and Liver Antioxidant Capacity of Lipopolysaccharide-Stressed Meat Rabbits
关键词：
yeast peptide%meat rabbits%lipopolysaccharide%inflammation%antioxidant capacity
摘 要：
The purpose of this study was to investigate the effects of yeast peptide on the growth performance of meat rabbits and serum cytokines and liver antioxidant capacity of lipopolysaccharide-stressed meat rabbits.The trial was divided into two phases.Phase 1:a total of 160 healthy 35-day-old weaned Hyla line rabbits were selected with an initial body weight of(0.96±0.06)kg,and randomly divided into 4 groups(half male and half female),with 8 replicates per group and 5 rabbits per replicate.Rabbits in control group(CON group)and lipopolysaccharide group(LPS group)fed a basal diet,and those in yeast peptide group(YP group)and yeast peptide remission group(L-YP group)fed the basal diet supplemented with 100 mg/kg yeast peptide.The feeding experiment lasted for 35 days,including 7 days of pre-trial period and 28 days of experimental pe-riod.Data such as body weight and feed intake were recorded and growth performance indexes of meat rabbits during the phase 1 were calculated.Phase 2:at the end of phase 1,rabbits in the LPS group and L-YP group were intramuscularily injected with 200 μg/kg BW LPS,and rabbits in the CON group and YP group were in-jected with the same amount of normal saline.The samples were collected 4 h after injection with LPS or nor-mal saline,and serum cytokine content,liver antioxidant indexes and mRNA relative expression levels of an-tioxidant genes were measured.The results showed as follows:1)adding yeast peptide significantly increased the average daily gain and significantly decreased the feed to gain ratio of meat rabbits(P<0.05),but had no significant effects on the average daily feed intake,diarrhea rate and mortality rate(P>0.05).2)The contents of serum interleukin-6(IL-6),interleukin-8(IL-8)and reactive oxygen species(ROS)were significantly de-creased by adding yeast peptide(P<0.05);lipopolysaccharide stress significantly increased the contents of ser-um tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,IL-8,transforming growth factor-β(TGF-β)and ROS of meat rabbits(P<0.05);serum TNF-α,IL-6,IL-10 and TGF-β contents had signifi-cant interactions between yeast peptide and lipopolysaccharide(P<0.05).Under lipopolysaccharide stress,adding yeast peptide significantly decreased the serum TNF-α and IL-6 contents of lipopolysaccharide-stressed meat rabbits(P<0.05).3)Adding yeast peptide significantly increased the liver superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activities and total antioxidant capacity(T-AOC)of meat rabbits(P<0.05),and significantly decreased the liver malondialdehyde(MDA)content(P<0.05);lipopolysac-charide stress significantly decreased the liver T-AOC and GSH-Px activity(P<0.05);SOD activity and SOD mRNA relative expression level in liver had a significant interaction between yeast peptide and lipopolysaccha-ride(P<0.05).Under lipopolysaccharide stress,adding yeast peptide significantly increased the liver SOD ac-tivity and SOD mRNA relative expression level of lipopolysaccharide-stressed meat rabbits(P<0.05).In sum-mary,adding 100 mg/kg yeast peptide to the diet can improve the growth performance of meat rabbits,and ef-fectively relieve the immune stress response of meat rabbits induced by lipopolysaccharide by reducing the level of inflammation and improving the antioxidant capacity.[Chinese Journal of Animal Nutrition,2024,36(8):5308-5318]
