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当前位置: 首页 > 文章 > 复合生物保鲜剂结合冰温贮藏对红鳍东方鲀的保鲜效果 水产学报 2019 (3) 688-696
Position: Home > Articles > Preservation effect of complex biological preservative combined with ice temperature storage on Takifugu rubripes Journal of Fisheries of China 2019 (3) 688-696

复合生物保鲜剂结合冰温贮藏对红鳍东方鲀的保鲜效果

作  者：
苏红;申亮;毕诗杰;张晓梅;郭芮;刘红英
单  位：
河北农业大学食品科技学院;河北农业大学海洋学院
关键词：
红鳍东方鲀;生物保鲜剂;保鲜;响应面法;柠檬酸;百里酚;海藻酸钠
摘  要：
为了延长红鳍东方鲀鱼块的货架期,利用响应面Box-Behnken原理设计柠檬酸、百里酚和海藻酸钠3因素3水平响应面分析实验,建立以菌落总数为响应值的二次多项式回归模型。并通过pH、汁液流失率、挥发性盐基氮(TVB-N)、硫代巴比妥酸(TBA)、菌落总数及优势腐败菌假单胞菌等指标测定,对最佳配比的复合生物保鲜剂的保鲜效果进行了验证。结果显示,冰温下,柠檬酸、百里酚、海藻酸钠的最优配比分别为0.68%、1.45%、1.94%,贮藏30 d时菌落总数为最低值5.50 lg (CFU/g),并且3种保鲜剂两两间均存在极显著的交互作用。经验证,该复合生物保鲜剂可以显著抑制微生物的增长,有效降低贮藏过程中的TVB-N含量、TBA含量,显著减缓了鱼块的腐败变质发生,货架期较对照组的15 d延长至30 d。
译  名：
Preservation effect of complex biological preservative combined with ice temperature storage on Takifugu rubripes
作  者：
SU Hong;SHEN Liang;BI Shijie;ZHANG Xiaomei;GUO Rui;LIU Hongying;College of Food Science and Technology, Hebei Agricultural University;Ocean College of Hebei Agricultural University;
单  位：
SU Hong%SHEN Liang%BI Shijie%ZHANG Xiaomei%GUO Rui%LIU Hongying%College of Food Science and Technology, Hebei Agricultural University%Ocean College of Hebei Agricultural University
关键词：
Takifugu rubripes;;complex biological preservative;;preservation;;response surface methodology;;citric acid;;thymol;;sodium alginate
摘  要：
The aim of this paper was to optimize complex biological preservatives consisting of citric acid, thymol and sodium alginate using three-variable, three-level Box-Behnken design to prolong the shelf life of Takifugu rubripes. A quadratic polynomial regression model was established with total plate counts as the response value.The preservation effects of complex biological preservatives were verified by measuring pH, drip loss, TVB-N,TBA, total plate counts and Pseudomonas sp.. The result showed that the optimal ratio of the complex biological preservatives at ice temperature is as follows: 0.68% citric acid, 1.45% thymol and 1.94% sodium alginate. In that condition, the lowest total plate count was 5.50 lg(CFU/g) on the 30 th day. What's more, citric acid and thymol,citric acid and sodium alginate, as well as thymol and sodium alginate had a significant interactive effect. The optimal complex biological preservatives could significantly inhibit the growth of microorganisms, and reduce the contents of TVB-N and TBA. It was proved that the complex biological preservatives could effectively delay the deterioration of T. rubripes, prolonging the shelf life of 15 d to 30 d.

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