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Position: Home > Articles > Progress on Baculovirus Surface Display System Progress in Veterinary Medicine 2014 (6) 107-113

杆状病毒表面展示系统研究进展

作  者:
吕慧芳;郭保党;张澍;姚伦广
单  位:
南阳师范学院;中英南阳洛桑昆虫生物学联合实验室;河南省伏牛山昆虫生物学重点实验室
关键词:
杆状病毒;表面展示;融合表达;囊膜糖蛋白
摘  要:
杆状病毒表面展示系统是近年发展起来的一种新型真核生物表面展示系统。目的蛋白或多肽与杆状病毒表面囊膜糖蛋白gp64融合表达或与特异的锚定部位结合,从而被展示于杆状病毒表面,能够很好地保持外源蛋白的活性。杆状病毒表面展示系统由于具有糖基化、磷酸化等翻译后修饰能力,而被广泛的应用于新型疫苗研制、基因转导与基因治疗、展示复杂的真核蛋白、构建多肽文库和抗体库以及单克隆抗体的制备等领域。论文对杆状病毒表面展示系统的研究现状进行了总结和阐述,并对其发展和应用前景做了展望。
译  名:
Progress on Baculovirus Surface Display System
作  者:
L Hui-fang;GUO Bao-dang;ZHANG Shu;YAO Lun-guang;Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect Biology,Henan Provincial Key Laboratory of Funiu Mountain Insect Biology,Nanyang Normal University;
关键词:
Baculovirus;;surface display;;fused expression;;glycoprotein
摘  要:
Baculovirus surface display system is developed in recent years as a new eukaryotic surface display system.Target protein or polypeptide fused with baculovirus surface glycoprotein gp64 or a combination of the specific anchor position,which was displayed and expressed on the surface of baculovirus virus,then maintained a good protein activity.Bacause of the ability of glycosylation,phosphorylation and other post translation modifications,baculovirus surface display system is widely used in the new vaccine,gene transfer and gene therapy,display of complex eukaryotic protein,construction of peptide library and antibody library and monoclonal antibody preparation etc.This article summarized and described the research status of baculovirus surface display system,and the prospect of development and application.

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