当前位置: 首页 > 文章 > 高效液相色谱-示差折光测定口香糖中山梨糖醇和麦芽糖醇的含量 安徽农学通报 2017 (17) 127-129
Position: Home > Articles > Separation and Identification of Sorbitol and Maltitol in Chewing Gum by High Performance Liquid Chromatography-refractive Index Detector Anhui Agricultural Science Bulletin 2017 (17) 127-129

高效液相色谱-示差折光测定口香糖中山梨糖醇和麦芽糖醇的含量

作  者:
何红春;李红梅
单  位:
宝应县农产品质量检测监督站;宝应县有机食品质量监督检验中心
关键词:
口香糖;山梨糖醇;麦芽糖醇;高效液相色谱;示差折光检测
摘  要:
目的:建立一种高效液相色谱-示差折光测定口香糖中山梨糖醇和麦芽糖醇含量的实验方法。方法:色谱柱采用Waters NH2柱(250mm×4.6mm,5μm),以乙腈∶水=70∶30为流动相,采用示差折光检测器测定口香糖中山梨糖醇和麦芽糖醇的含量。结果:采用本实验检测方法,山梨糖醇和麦芽糖醇的线性范围为1.6~6.4mg/mL,该方法的最小检出限0.1mg/mL,加标回收率为92.46%~101.08%,方法的相对标准偏差0.88%~1.96%。结论:该方法精密度好、回收率和准确度高,适用于口香糖中山梨糖醇和木糖醇的定量分析。
译  名:
Separation and Identification of Sorbitol and Maltitol in Chewing Gum by High Performance Liquid Chromatography-refractive Index Detector
作  者:
He Hongchun;Baoying County Agricultural Product Quality Inspection and Supervision Station;
关键词:
Chewing gum;;Sorbitol;;Maltitol;;HPLC;;Refractive index detector
摘  要:
Objective]A high performance liquid chromatography method for separation and determination of sorbitol and maltitol in chewing gum with sticky rice was established.[Method]The analytical column was Waters NH2(250mm×4.6mm,5μm),the mobile phase was acentonitrile-water(70∶30,V/V),the samples were determined by an refractive index detector detector.[Result]The calibration curves had good linearity in the range of 1.6~6.4mg/mL for sorbitol and maltitol,the detection limit for sorbitol and maltitol was 0.1mg/mL,the average recoveries of sorbitol and maltitol were 92.46% ~101.08% ,RSD=0.88% ~1.96% .[Conclusion]The method was a convenient,rapid and effective method for qualitatively analysis of sorbitol and maltitol in chewing gum.

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