当前位置: 首页 > 文章 > 17β-雌二醇-ARIS构建及其标准曲线的质量评估 动物医学进展 2001,22 (2) 79-81
Position: Home > Articles > Plotting Standard Curves for 17β Estradiol by ARIS and Its Quality Analysis Progress in Veterinary Medicine 2001,22 (2) 79-81

17β-雌二醇-ARIS构建及其标准曲线的质量评估

作  者:
周乐;史远刚;李键;王建辰
单  位:
西北农林科技大学生命学院
关键词:
17β-雌二醇;酶免疫测定法;ARIS;葡萄糖氧化酶;黄素腺嘌呤二核苷酸
摘  要:
以 1 7β-雌二醇 -黄素腺嘌呤二核苷酸结合物为标记物 ,以葡萄糖氧化酶为全酶 ,建立了 1 7β-雌二醇脱辅基酶再激活免疫测定系统 (1 7β- E2 - ARIS) ,完成了两条标准曲线的制作 ,通过 logit代换法对两条标准曲线进行了直线回归。两条标准曲线的拟合度 (r)分别为0 .996 7* * 和 0 .9991 * * ,灵敏度分别为 1 1 .5 4pg· m L- 1 (2 . 31 pg·孔 - 1 )和 1 4.88pg·m L- 1 (2 . 98pg·孔 - 1 ) ,平均变异系数(CV% )分别为 4.6 4%和 9.34% ,测定范围为 31 .2 5~ 1 0 0 0 pg·m L- 1 。
译  名:
Plotting Standard Curves for 17β Estradiol by ARIS and Its Quality Analysis
作  者:
ZHOU Le,SHI Yuan gang,LI Jian,WANG Jia chen (College of Life Science,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)
关键词:
7β-estradiol;enzyme immunoassay;ARIS;glucose oxidase;flavin adenine dinucleotide
摘  要:
Apoenzyme reactivation immunoassay system for 17β estradiol was constructed with 17β estradiol flavin adenine dinucleotide conjugate as label and with glucose oxidase as holoenzyme。Two standard curves were obtained,and linerized by logitic trasmission。The regression cofficients are 0.9967 and 0.9991(n=7) resp.,sensitivity 11.54 pg·mL -1 (2.31 pg·well -1 ) and 14.88 pg·mL -1 (2.98 pg·well -1 )resp.,mean CV% 4.64 and 9.34 resp.,and the range of determination is from 31.25 to 1 000 pg·mL -1 。

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊