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Position: Home > Articles > Study on the Polymorphism of Leptin Exon 2's E2JW and E2FB Locus in Xinjiang Brown Cattle Journal of Xinjiang Agricultural University 2009,32 (5) 6-9

新疆褐牛leptin基因第二外显子E2JW和E2FB位点的多态性分析

作  者:
姜长生;张杨;孙亚伟;张彩芳;雷程红;颜庭生;姚刚
单  位:
新疆农业大学;新疆塔城地区种牛场;新疆畜牧科学院
关键词:
瘦素基因;外显子2;多态性
摘  要:
利用改进的DNA提取方法和PCR-RFLP技术,对145头新疆褐牛leptin基因外显子2上E2JW和E2FB两个位点的多态性进行了分析。结果表明,新疆褐牛leptin基因外显子2上E2JW位点存在CIaⅠ限制性内切酶的酶切位点,E2FB位点存在Kpn2Ⅰ限制性内切酶的酶切位点。在这2个酶切位点上均有多态性。其中,E2JW位点AA基因型频率为0.81,AB型为0.19,BB型为0;A等位基因频率为0.91,B等位基因频率为0.09。E2FB位点CD型基因型频率为0.96,DD型为0.04,CC型为0;C等位基因频率为0.48,D等位基因频率为0.52。
译  名:
Study on the Polymorphism of Leptin Exon 2's E2JW and E2FB Locus in Xinjiang Brown Cattle
作  者:
JIANG Chang-sheng1,ZHANG Yang2,SUN Ya-wei3,ZHANG Cai-fang1,LEI Cheng-hong3,YAN Ting-sheng4,YAO Gang3(1.College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;2.Xinjiang Academy of Animal Science,Urumqi 830000,China;3.College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,China;4.Xinjiang Herd Bull Farm of Tacheng Area,Tacheng 834700,China)
关键词:
leptin gene;exon 2;polymorphism
摘  要:
The polymorphism of leptin exon 2's E2JW and E2FB locus from blood samples of 145 Xinjiang brown cattle were analyzed with the modified DNA extracting method and PCR-RFLP.The results showed that there were CIaⅠ endonuclease site on E2JW and Kpn2Ⅰ endonuclease site on E2FB of leptin gene exon 2 locus in Xinjiang brown cattle,and the analysis of polymorphism showed that frequency of AA,AB,BB genotype were 0.81,0.19,0;A,B allele frequency were 0.91,0.09 on E2JW locus;CD site frequency of CD,DD,CC genotype were 0.96,0.04,0,C,D allele frequency were 0.48,0.52 on E2FB locus.

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