作 者：
孙学亮;杨树元;陈书奇;孟凡亮;陈成勋;郭永军;于宏
关键词：
复方中草药;白点鲑;亚东鲑;抗氧化;非特异性免疫;消化酶
摘 要：
在水温10～12℃下,将体质量(224.14±26.73)g的白点鲑Salvelinus pluvius和体质量(152.12±7.46)g的亚东鲑Salmo trutta fario饲养在直径1m、水深0.7m的玻璃钢材质圆桶中,在基础饲料中添加0.5%的复方中草药(大黄、黄芪、连翅、黄苓和丹皮)进行投喂,在投喂0d、7d、14d、21d和28d时从鱼尾柄腹侧用肝素处理的容器抽血采样,测定血液中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)、肠道淀粉酶(AMS)、脂肪酶(LPS)和蛋白酶活性,及丙二醛(MDA)和一氧化氮(NO)含量,研究复方中草药对鱼血液抗氧化能力、非特异性免疫力及消化酶活力的影响。结果表明:摄食含复方中草药饲料14d时的亚东鲑血液T-AOC活力和SOD活力显著高于0d(P<0.05),MDA含量和CAT活力各组间差异不显著(P>0.05);7d和14d的亚东鲑血液AKP活力和ACP活力显著高于其他组(P<0.05),14d时NO浓度和LZM活性与0d差异显著(P<0.05);肠道蛋白酶活力差异不显著(P>0.05),淀粉酶活力和脂肪酶活力在14d时在显著高于其他组(P<0.05)。摄食含复方中草药饲料14d的白点鲑血液T-AOC活力显著高于其他组(P<0.05),MDA含量和SOD活力差异不显著(P>0.05),21d时CAT活力显著高于其他组(P<0.05);AKP活力差异不显著(P>0.05),7d、14d时ACP活力和NO浓度显著高于其他组(P<0.05),21d时LZM含量显著高于其他组(P<0.05);14 d时淀粉酶活力显著高于其他组(P<0.05),肠道蛋白酶活力和脂肪酶活力差异不显著(P>0.05)。综上所述,摄食含复方中草药的饲料,对白点鲑、亚东鲑血液的抗氧化性、非特异性及消化酶指标有促进作用,投喂14 d时的效果最佳。
译 名：
Effects of Compound Chinese Herbal Medicine on Antioxidant, Non-specific Immunity and Digestive Enzyme Activity in Salvelinus pluvius and Salmo trutta fario
作 者：
SUN Xueliang;YANG Shuyuan;CHEN Shuqi;MENG Fanliang;CHEN Chengxun;GUO Yongjun;YU Hong;Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Sciences, Tianjin Agricultural University;Yangliuqing Agriculture Service Center in Xiqing Area of Tianjin City;Fengpei Aquatic Products Co., Ltd.;
关键词：
compound Chinese herbal medicine;;Salvelinus pluvius;;Salmo trutta fario;;anti-oxidation;;non-specific immunity;;digestive enzyme
摘 要：
At a water temperature of 10 ～ 12 ℃, the body mass(224.14±26.73)g of Salceinus pluvius and the body mass(152.12±7.46)g of Salmo trutta fario were raised in a FRP diameter drum with a diameter of 1 m and a water depth of 0.7 m. Feeding a basal feed supplemented with 0.5% compound Chinese herbal medicine(rhubarb, scutellaria, wing, scutellaria and paeonol), When feeding0 d, 7 d, 14 d, 21 d and 28 d, the ventral side of the fish stalk was sampled with a heparin-treated container to measure total antioxidant capacity(T-AOC), superoxide dismutase(SOD), catalase(CAT), acid phosphatase(ACP)in the blood, alkaline phosphatase(AKP), lysozyme(LZM), intestinal amylase(AMS), lipase(LPS)and protease activity, and malondialdehyde(MDA)and nitric oxide(NO)levels, study the effect of compound Chinese herbal medicine on blood antioxidant capacity, non-specific immunity and digestive enzyme activity of fish. The results showed that the blood T-AOC activity and SOD activity of Salmo trutta fario after eating containing compound Chinese herbal medicine for 14 days were significantly higher than 0 d(P < 0.05), and the difference between MDA content and CAT activity was not significant(P > 0.05). The AKP activity and ACP activity of Salmo trutta fario in 7 d and 14 d were significantly higher than those in other time periods(P < 0.05). At 14 d, NO concentration and LZM activity were significantly different from 0 d(P < 0.05); There was no significant difference in intestinal protease activity(P > 0.05). Amylase activity and lipase activity were significantly higher than other time periods at 14 d(P < 0.05). Amylase activity and lipase activity were significantly higher than other time periods at 14 d(P < 0.05). The T-AOC activity of Salvelinus pluvius after eating containing compound Chinese herbal medicine for 14 d was significantly higher than that of other time periods(P < 0.05). The difference of MDA content and SOD activity was not significant(P > 0.05). At 21 d, CAT activity was significantly higher than other periods(P < 0.05). AKP activity was not significantly different(P > 0.05), the difference of AKP activity was not significant(P > 0.05). At 7 d and 14 d, ACP activity and NO concentration were significantly higher than other periods(P < 0.05). At 21 d, LZM content was significantly higher than other periods(P < 0.05). At 14 d, amylase activity was significantly higher than other periods(P < 0.05), and there was no significant difference in intestinal protease activity and lipase activity(P > 0.05). In summary, feeding the feed containing compound Chinese herbal medicine has a promoting effect on the antioxidant, non-specific and digestive enzymes of the blood of Salvelinus pluvius and Salmo trutta fario. The effect is best when fed for 14 days.
