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Position: Home > Articles > Determination of Seven Ginsenosides in Stems,Leaves and Root of Panax ginseng C.A.Mey by HPLC-ELSD Special Wild Economic Animal and Plant Research 2009,31 (4) 54-55+60

HPLC-ELSD法测定人参茎、叶、根中7种人参皂苷含量

作  者:
孙成贺;王英平
单  位:
中国农业科学院特产研究所
关键词:
HPLC-ELSD;人参;人参皂苷
摘  要:
目的建立适合于人参茎、叶、根皂苷的高效液相色谱-蒸发光散射检测方法(HPLC-ELSD),同时测定人参茎、叶、根中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rd的含量。方法HPLC-ELSD方法,色谱柱:SHIMADZUC185μm(4.6×250mm),流动相:乙腈-水,梯度洗脱。漂移管温度70℃,载气流速350mi/min。结果人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rd分别在0.462~6.48μg、0.678~4.068μg、0.627~6.144μg、0.247~5.48μg、0.378~6.068μg、0.227~6.844μg、0.561~6.88μg呈良好的线性关系。结论该法准确、重现性好,适于人参茎、叶、根皂苷的定量分析。
译  名:
Determination of Seven Ginsenosides in Stems,Leaves and Root of Panax ginseng C.A.Mey by HPLC-ELSD
作  者:
SUN Cheng-he,WANG Ying-ping(Institute of Special Wild Economic Animal and Plant Science,CAAS.,Jilin132109,China)
关键词:
HPLC-ELSD;Panax ginseng;Ginsenoside
摘  要:
Aim establish a HPLC-ELSD method for the determination of ginsenosides Rg1,Re,Rf,Rbl,Rc,and Rd in the stems,leaves and roots of Panax ginseng,simultaneous detect its content.Method HPLC-ELSD method,column:SHIMADZU C18 5μm(4.6×250mm),mobile phase acetonitrile and water,gradient elution.drift tube temperature70℃,flow rate of carrier gas 350mi/min.Result:There was a good linear relationship among ginsenosides Rg1、Re、Rf、Rb1、Rc、Rb2、Rd in 0.462~6.48 μg,0.678~4.068 μg,0.627~6.144 μg,0.247~5.48 μg,0.378~6.068 μg,0.227~6.844 μg,0.561~6.88 μg respectively.Conclusion this method is accurate and good reproducibility suitable for the measurement of ginsenosides in the stems,leaves and roots of ginseng.

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