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当前位置: 首页 > 文章 > 根癌农杆菌介导的淡紫拟青霉遗传转化体系的建立 林业科学 2010,46 (10) 97-104
Position: Home > Articles > Establishment of Agrobacterium tumefaciens-Mediated Transformation System of Paecilomyces lilacinus Scientia Silvae Sinicae 2010,46 (10) 97-104

根癌农杆菌介导的淡紫拟青霉遗传转化体系的建立

作  者：
王曦茁;朴春根;李虹;汪来发;郭民伟;李永;刘晓莉
单  位：
中国林业科学研究院森林生态环境与保护研究所;安徽农业大学林学与园林学院;中国林业科学研究院林业研究所
关键词：
根癌农杆菌;淡紫拟青霉;G-418;遗传转化
摘  要：
基于根癌农杆菌介导的遗传转化方法,选择植物线虫生防真菌淡紫拟青霉的分生孢子为受体,建立以G418为筛选标记遗传转化体系。通过优化遗传转化条件,达到较高的转化效率:1000～2400个转化子·10-6分生孢子。对转化子进行PCR验证,表明T-DNA已整合到淡紫拟青霉的基因组中,表型测定发现转化子的遗传表现稳定。农杆菌介导淡紫拟青霉突变体库的建立,为进一步筛选对植物线虫致病性高的突变体、了解淡紫拟青霉的侵染过程和侵染机制提供基础,对培育更优良的植物线虫生防菌株,开发高效生防制剂等具有重要的意义。
译  名：
Establishment of Agrobacterium tumefaciens-Mediated Transformation System of Paecilomyces lilacinus
作  者：
Wang Xizhuo1 Piao Chungen1 Li Hong2 Wang Laifa1 Guo Minwei1 Li Yong1 Lui Xiaoli3(1. Key Laboratory of Forest Protection,State Forestry Administration Research Institute of Forest Ecology,Environment and Protection,CAF Beijing 100091;2. Research Institute of Forestry,CAF Beijing 100091;3. School of Forestry and Landscape Architecture,Anhui Agricultural University Hefei 230036)
关键词：
Agrobacterium tumefaciens;Paecilomyces lilacinus;G-418;transformation
摘  要：
In this study,the research is conidia of Paecilomyces lilacinus,a pathogenic fungus of plant nematodes,was selected as the receptor. An efficient genetic transformation system of P. lilacinus was established by harboring G-418 resistance cassette as a selective marker to construct vector pBI-G3C-aphI based on Agrobacterium tumefaciens-mediated transformation. Using this system,P. lilacinus was successfully transformed with an efficiency of 1 000-2 400 transformants per 106 spores. It indicated that T-DNA had been integrated into the genome of P. lilacinus by PCR analysis on the transformants,and the genetic character of transformants remained stable. The establishment of T-DNA insertional mutants library would provides a basis for further selecting pathogenic mutants,and understanding pathogenisis of P. lilacinus,which is of great significance to foster a better anti-nematode strain and develop effective biocontrol agents.

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