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Position: Home > Articles > Determination of Carboxypeptidase Inhibitor Eluted from Potatoes by RP-HPLC FOOD SCIENCE 2005,26 (3) 195-197

反相高效液相色谱法测定土豆羧肽酶抑制剂

作  者:
唐瑜菁;陈蕰;金坚
单  位:
工业生物技术教育部重点实验室江南大学生物工程学院
关键词:
反相高效液相色谱;土豆羧肽酶抑制剂;土豆
摘  要:
建立土豆羧肽酶抑制剂的反相高效液相色谱测定方法。采用80%乙醇进行样品前处理,上C8柱,以乙腈-水为流动相作梯度洗脱,检测波长280nm。土豆羧肽酶抑制剂在35%~40%的乙腈区间内被洗脱下来,在10~1000mg/L时,其峰面积与浓度呈良好的线性关系,R2=0.9982,加标回收率101.3%。该方法适用于土豆及其它植物粗提液中土豆羧肽酶抑制剂的定量分析及分离纯化过程的监控。
译  名:
Determination of Carboxypeptidase Inhibitor Eluted from Potatoes by RP-HPLC
作  者:
TANG Yu-jing,CHEN yun,JIN Jian* (Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, China)
关键词:
reverse phase high performance liquid chromatography (RP-HPLC);carboxypeptidase inhibitor from potato (CPI);potato
摘  要:
A RP-HPLC method was established for the determination of Carboxypeptidase Inhibitor from Potatoes (CPI) .The operation was carried out by applying the sample treated with 80% ethanol a C8 column with the mobile phase of water (with 0. 1%TCA)-acetonitrile (with 0.1%TCA)gradient, monitored at 280nm. CPI was eluted at 35%~40% acetonitrile (V/V), and had a good linearity at the range of 10mg/L to 1000mg/L with R2=0.9982 .The recovery CPI of this method 101.3% . This method has been successfully applied to analysis CPI from various materials and process control during CPI purification.

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