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当前位置: 首页 > 文章 > PITC柱前衍生-反相高效液相色谱法测定黄酒中游离氨基酸和生物胺 食品科学 2016,37 (8) 159-163
Position: Home > Articles > Simultaneous Determination of Free Amino Acids and Biogenic Amines in Chinese Rice Wine by RP-HPLC with Pre-Column Phenylisothiocyanate(PITC)Derivatization FOOD SCIENCE 2016,37 (8) 159-163

PITC柱前衍生-反相高效液相色谱法测定黄酒中游离氨基酸和生物胺

作  者：
芮鸿飞;张晓瑜;刘兴泉;吴峰华;王卓然;李凯利
单  位：
浙江农林大学农业与食品科学学院
关键词：
黄酒;氨基酸;生物胺;反相高效液相色谱
摘  要：
建立柱前衍生-反相高效液相色谱,同时测定黄酒中17种氨基酸及7种生物胺含量的方法。采用异硫氰酸苯酯柱前衍生试剂,采用Inertsil ODS-SP C_(18)(250 mm×4.6 mm,5μm)分析,以乙酸钠溶液和乙腈溶液混合液进行梯度洗脱,在254 nm波长处进行氨基酸和生物胺的含量分析。氨基酸和生物胺在2.0~500 mg/L和0.5~125 mg/L范围内具有良好的线性关系,相关系数R~2大于0.99,平均回收率在86.44%~99.75%之间,相对标准偏差在0.31%~3.53%之间;检出限为0.02~0.25 mg/L,定量限为0.25~4.50 mg/L。方法线性范围广、准确性高、稳定性好,适用于黄酒中氨基酸及生物胺的检测。
译  名：
Simultaneous Determination of Free Amino Acids and Biogenic Amines in Chinese Rice Wine by RP-HPLC with Pre-Column Phenylisothiocyanate(PITC)Derivatization
作  者：
RUI Hongfei;ZHANG Xiaoyu;LIU Xingquan;WU Fenghua;WANG Zhuoran;LI Kaili;School of Agriculture and Food Science,Zhejiang Agriculture and Forestry University;
关键词：
Chinese wice wine;;amino acids;;biogenic amines;;reversed-phase high performance liquid chromatography(RP-HPLC)
摘  要：
A reversed-phase high performance liquid chromatographic(RP-HPLC)method has been presented for the simultaneous determination of 17 free amino acids and 7 biogenic amines in Chinese rice wine.Wine samples were precolumn derivatized with phenylisothiocyanate(PITC),separated using Inertsil ODS-SP C_(18) chromatography column(250 mm ×4.6 mm,5 μm),eluted with a mobile phase composed of sodium acetate,acetonitrile and ultra-pure water,and detected at 254 nm.The calibration curves of 17 amino acids and 7 biogenic amines all exhibited good linearity over the range from 2.0 to 500 mg/L and 0.5 to 125 mg/L,respectively.Mean recoveries for amino acids and biogenic amines were between 86.44%and 99.75%,and the relative standard deviations(RSDs)were in the range of 0.31%–3.53%.The limits of detection(LODs)for 17 free amino acids and 7 biogenic amines were in the range of 0.02–0.25 mg/L,and the limits of quantification(LOQs)were 0.25–4.50 mg/L.The method is stable,accurate,and suitable for the simultaneous determination of amino acids and biogenic amines in Chinese rice wine,having a wide linear range.

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