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Position: Home > Articles > Optimization of enzymatic modification conditions of albumin edible film by response surface methodology Science and Technology of Food Industry 2016,37 (18) 317-323

响应面法优化可食性鸡蛋清蛋白膜的酶法改性工艺

作  者:
郑优;贾亮;段蓉;马莹莹
单  位:
西南大学荣昌校区
关键词:
鸡蛋清蛋白;谷氨酰胺转移酶;可食膜
摘  要:
采用响应面分析法(RSM)对可食性鸡蛋清蛋白膜的酶法改性工艺进行优化。以谷氨酰胺转移酶(TG-B)浓度、p H、注模前酶作用时间及干燥温度为单因素,对膜的机械性能(拉伸强度、断裂伸长率)和阻隔性能(水蒸气透过系数、透油系数)进行测定,得出各指标的二次回归模型。结果表明,TG-B酶浓度0.13%、p H8.34、注模前酶作用时间45 min、干燥温度20℃时膜的各项性能最佳。该工艺参数下膜各性能指标分别为:拉伸强度(5.225±0.108)MPa、断裂伸长率70.492%±3.172%、水蒸气透过系数(3.405±0.067)g·mm/(m2·d·k Pa)、透油系数(0.812±0.007)g·mm/(m2·d)。
译  名:
Optimization of enzymatic modification conditions of albumin edible film by response surface methodology
作  者:
ZHENG You;JIA Liang;DUAN Rong;MA Ying-ying;Rongchang Campus,Southwest University;
关键词:
egg albumin;;tranglutaminase;;edible film
摘  要:
The enzymatic modification conditions of albumin edible film were optimized through response surface methodology. With TG- B enzyme concentration,p H value,enzyme reaction time,drying temperature being response factors,the tensile strength,elongation,water vapor permeability and oil permeability of edible film were measured and the quadratic regression models of indicators were achieved. The results showed that the optimal enzymatic modification conditions were TG- B enzyme concentration 0.13%,p H value 8.34,enzyme reaction time45 min and drying temperature 20 ℃.Under this condition,the properties of edible films were tensile strength( 5.225± 0.108) MPa,tensile strength 70.492%± 3.172%,water vapor permeability( 3.405 ± 0.067) g·mm /( m2·d·k Pa),oil permeability( 0.812 ± 0.007) g·mm /( m2·d).

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