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当前位置: 首页 > 文章 > 虎舌红组织培养及快速繁殖技术体系研究 安徽农业科学 2009,37 (13) 69-70
Position: Home > Articles > Study on the Technique System of Tissue Culture and Rapid Propagation of Ardisia mamillata Journal of Anhui Agricultural Sciences 2009,37 (13) 69-70

虎舌红组织培养及快速繁殖技术体系研究

作  者：
曾云英;孙英
单  位：
九江学院土木工程与城市建设院
关键词：
虎舌红;组织培养;快速繁殖
摘  要：
[目的]寻找快速获得整齐一致的虎舌红苗木的方法,为虎舌红的产业化生产提供理论与技术支撑。[方法]以虎舌红当年生枝条和带芽茎段为外植体进行组织培养试验。[结果]诱导虎舌红芽分化的最优激素组合是TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO33.0mg/L,其平均诱导率达94.56%;其次是TDZ 0.5 mg/L+NAA 0.4 mg/L+AgNO35.0 mg/L,其平均诱导率为89.18%;2号和3号培养基上的芽分化率最低,不到35%。诱导虎舌红芽增殖的最佳激素组合是6-BA 1.5 mg/L+NAA 0.5 mg/L,其平均增殖系数为5.14。IBA对虎舌红生根的影响比NAA大,培养基1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L诱导虎舌红生根的效果较好,生根率达75.8%。虎舌红组培幼苗的移栽成活率达87.6%。[结论]诱导虎舌红芽分化的最佳培养基为1/2 MS+TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO33.0 mg/L,最佳增殖培养基为1/2 MS+6-BA 1.5 mg/L+NAA 0.5 mg/L,最佳生根培养基为1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L。
译  名：
Study on the Technique System of Tissue Culture and Rapid Propagation of Ardisia mamillata
作  者：
ZENG Yun-ying et al (College of Civil Engineering and Urban Construction,Jiujiang University,Jiujiang,Jiangxi 332005)
关键词：
Ardisia mamillata;Tissue culture;Rapid propagation
摘  要：
[Objective] The aim of the study was to find a method of getting uniform Ardisia mamillata seedlings rapidly and provide theoretical and technical support for its industrial production.[Method] With current-year branches and stem sections with buds of A.mamillata as explants,the experiment on its tissue culture was performed.[Result] The optimum hormone combination for inducing the bud differentiation of A.mamillata was TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO3 3.0 mg/L and its average induction rate was up to 94.56%;TDZ 0.5 mg/L+NAA 0.4 mg/L+AgNO3 5.0 mg/L was secondary and its average induction rate was 89.18%.The bud differentiation rate was lowest on media 2 and 3,which was less than 35%.The optimum hormone combination for inducing the bud multiplication of A.mamillata was 6BA 1.5 mg/L+NAA 0.5 mg/L and its average multiplication coefficient was 5.14.The effect of IAA on the rooting of A.mamillata was greater than that of NAA.The effect on inducing the rooting of A.mamillata of medium 1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L was better and its rooting rate was up to 75.8%.The survival rate of transplanted A.mamillata seedlings from tissue culture was up to 87.6%.[Conclusion] The optimum medium for inducing the bud differentiation of A.mamillata was 1/2 MS+TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO3 3.0 mg/L,that for inducing its bud multiplication was 1/2 MS+6-BA 1.5 mg/L+NAA 0.5 mg/L and that for inducing its rooting was 1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L.

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