中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 虎舌红组织培养中有效无菌材料的获得研究 安徽农业科学 2012,40 (11) 67-68
Position: Home > Articles > Research on Obtaining Effective Non-bacteria Material of Ardisia mamillata Hance among Tissue Culture Journal of Anhui Agricultural Sciences 2012,40 (11) 67-68

虎舌红组织培养中有效无菌材料的获得研究

作  者：
曾云英
单  位：
江苏广播电视大学城市科学系
关键词：
虎舌红;组织培养;无菌材料
摘  要：
[目的]探索获得虎舌红(Ardisia mamillata Hance)组织培养中有效无菌材料的途径和方法。[方法]以虎舌红为试材,研究了外植体母本的预处理和外植体的采集、灭菌、接种等处理方式对获得有效无菌材料的影响。[结果]2月将母株移入室内,进行预处理,3月中旬取当年生枝条和萌蘖条上的茎尖,用去污剂(肥皂水或洗衣粉水)浸泡并刷洗后,流水冲洗1～2 h,用75%乙醇表面消毒30 s,无菌水冲洗2～3次后放入0.1%升汞中灭菌(先用0.1%升汞消毒4 min,无菌水冲洗3次,再用0.1%升汞消毒4 min,无菌水冲洗5～8次)后进行有效接种,这种方法能有效降低污染率,提高成活率。[结论]该研究可为进一步研究和大规模生产虎舌红提供技术支持。
译  名：
Research on Obtaining Effective Non-bacteria Material of Ardisia mamillata Hance among Tissue Culture
作  者：
ZENG Yun-ying(City Science College,Jiangsu Radio & TV University,Nanjing,Jiangsu 210036)
关键词：
Ardisia mamillata Hance;Tissue culture;Non-bacteria material
摘  要：
[Objective] The purpose was to explore the way of obtaining effective non-bacteria material of Ardisia mamillata Hance among tissue culture.[Method] The effects of pretreating of explant female parent and explant collecting,sterilizing and inoculating etc on obtaining effective non-bacteria material of Ardisia mamillata Hance.[Result] The female plant is pretreated in the door in February,then stem tips of current year's branches and sprouting branches are collected in mid-March and they are cleaned with eradicator,then are washed by the tap water for one or two times.Explants are sterilized in 70% alcohol for 30 seconds,and are sterilized in 0.1% mercuric chloride(firstly sterilized in 0.1% mercuric chloride for 4 minutes,secondly sterilized in 0.1% mercuric chloride for 4 minutes after washed by the tap water for three times,finally washed by the tap water for 5-8 times) after washed 2-3 times by using sterile water.This way can effectively decreased pollution rate and increased survival rate.[Conclusion] The study provides a technological support for further researching and producing in a large scale of Ardisia mamillata Hance.

相似文章

导出引用
计量
文章访问数: 18
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46