当前位置: 首页 > 文章 > NTG诱变筛选高产柚苷酶抗药性突变株 江西农业大学学报 2007,29 (4) 162-166
Position: Home > Articles > Screening of Chemical Resistant Mutant Strains with High Naringinase-producing Capacity by Means of NTC Induction Acta Agriculturae Universitatis Jiangxiensis 2007,29 (4) 162-166

NTG诱变筛选高产柚苷酶抗药性突变株

作  者:
陈玲;涂晓嵘;涂国全
单  位:
江西农业大学生物科学与工程学院
关键词:
柚苷酶;抗药性致死突变;透明圈
摘  要:
通过筛选柚苷酶产生菌3-54菌株孢子的敏感抗生素最低致死浓度,采用NTG对出发菌株孢子进行诱变处理,将诱变后的孢子涂在含纳他霉素致死浓度(2.0 U/mL)的平板筛选培养基上,获得了大量的抗药性致死突变株。再结合透明圈法,经过透明圈初筛和多次摇瓶复筛,从180个纳他霉素抗性基因突变株中筛选得到1株摇瓶发酵单位达770.06 U/mL的3-54-NTG-16号菌株,比出发菌株的产酶能力提高了近100%。
译  名:
Screening of Chemical Resistant Mutant Strains with High Naringinase-producing Capacity by Means of NTC Induction
作  者:
CHEN Ling,TU Xiao-rong,TU Guo-quan(Department of Bioengineering,JAU,Nanchang 330045,China)
关键词:
naringinase;chemical resistant lethal mutation;transparent circle
摘  要:
After testing the resistance of sensitive antibiotic to naringinase-producing strains NO.3-54,a lot of chemical resistant mutants were screened after the spores regenerated on the lethal media when they were treated with NTG.Through the transparent circle screening and sever rotation-flask experiments,the high-yielding strain 3-54-NTG-16 was obtained from the 180 mutants,the productivity of which is 770.06U/mL,almost 100% higher than the original strain's in the rotation-flask experiments.

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