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Position: Home > Articles > Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spec-trometry for Bacterial Strain Identification Journal of Agricultural Catastrophology 2019,9 (5) 20-23

MALDI-TOF MS技术用于细菌鉴定的研究进展

作  者:
王淑娴;刁菁;樊英;李乐;叶海斌;刘洪军
单  位:
山东省海洋生物研究院病害与渔药研究中心
关键词:
MALDI-TOFMS;细菌鉴定;亚种水平;抗性机制
摘  要:
基质辅助激光解吸电离时间质谱(MALDI-TOF MS)技术已成为微生物实验室中快速有效的细菌物种鉴定新技术.常规的细菌鉴定主要基于生化测试并且需要冗长的孵育程序,相比之下,MALDI-TOF MS可以在几分钟内直接从新生菌落中鉴定出细菌种类.目前已经开发了基于MALDI-TOF MS技术用于细菌鉴定的多种方法,并且已经在多项研究中证明了该方法的有效性.该技术还适用于对生物亚种水平的分类以及某些抗生素抗性机制的表型检测,例如β-内酰胺酶的产生等.
译  名:
Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spec-trometry for Bacterial Strain Identification
作  者:
WANG Shu-xian;Disease and Fishery Medicine Research Centre,Mariculture Institute of Shandong Province;
关键词:
MALDI-TOF MS;;Bacteria identification;;Subspecies level;;Resistance mechanisms
摘  要:
Matrix-assisted laser desorption/ionization-time of flight mass spectrometer(MALDI-TOF MS) is one of the most popular MS instruments used in biology,due to its rapid and precise identification of genus and species. Conventional bacterial identi cation is based essentially on biochemical tests and requires lengthy incubation procedures. By contrast,MALDI-TOF MS can identify bacterial species in a few minutes,based on identifying a characteristic spectrum of each species and then matched with a large database within the instrument. Various approaches have been developed for bacterial identification and the efficacy of this method has been demonstrated in several studies.This technique should also be suitable for the classification of organisms to subspecies level and for the phenotypic detection of certain antibiotic resistance mechanisms, such as β-lactamases production.

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