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当前位置: 首页 > 文章 > 高效液相色谱-蒸发光散射法测定肉制品中山梨糖醇 肉类研究 2015 (8) 17-21
Position: Home > Articles > Determination of Sorbitol in Meat Products by High Performance Liquid Chromatography-Evaporation Light Scattering Detector MEAT RESEARCH 2015 (8) 17-21

高效液相色谱-蒸发光散射法测定肉制品中山梨糖醇

作  者：
甘凝岚
单  位：
江苏省产品质量监督检验研究院
关键词：
山梨糖醇;肉制品;高效液相色谱-蒸发光散射法
摘  要：
建立肉制品中山梨糖醇的高效液相色谱-蒸发光散射检测器(high performance liquid chromatographyevaporation light scattering detector,HPLC-ELSD)测定法。采用乙腈-水溶液(7:3,V/V)作为流动相直接超声提取样品,经Agilent ZORBAX NH_2(4.6 mm×250 mm,5μm)色谱柱分离,蒸发光散射检测器检测;优化ELSD检测器参数为飘移管温度90℃,氮气流速2.0L/min。结果表明:山梨糖醇含量在0.2~5.0mg/mL范围内有良好的线性,方法相对标准偏差(relative standard deviation,RSD)在1.28%~2.02%之间,回收率在88.3%~99.0%之间。本方法具有样品前处理简便、方法检出限低、方法重现性好的特点,适用于肉制品中山梨糖醇含量的测定。
译  名：
Determination of Sorbitol in Meat Products by High Performance Liquid Chromatography-Evaporation Light Scattering Detector
作  者：
GAN Ninglan;Jiangsu Product Quality Testing and Inspection Institute;
关键词：
sorbitol;;meat products;;high performance liquid chromatography-evaporation light scattering detector(HPLC-ELSD)
摘  要：
A high performance liquid chromatography with evaporative light-scattering detection(HPLC-ELSD) method was developed for the determination of sorbitol in meat products The samples were ultrasonically extracted using acetonitrile water(7:3,V/V) as mobile phase and separated on ZORBAX NH_2 Agilent column,then detected by evaporative light scattering detector(ELSD).Optimized ELSD detector parameters such as drift tube temperature and nitrogen flow rate were set at 90℃ and 2.0 L/min,respectively.The results showed that sorbitol displayed good linearity relationship over the concentration range of 0.2 to 10 mg/mL.The method precision(relative standard deviation,RSD) ranged from 1.28% to 2.02%,and the recovery was between 88.3% and 99.0%.This method has the advantages of simple sample pretreatment,low detection limit and good reproducibility.So it is useful for the determination of sorbitol in meat products.

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