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Position: Home > Articles > Study on Phenol-Sulfuric Acid Method for Determination of Polysaccharide Content in Pleurotus eryngii FOOD SCIENCE 2008,29 (12) 555-558

苯酚-硫酸法测定杏鲍菇多糖方法的研究

作  者:
郭金龙;陈有君;孙国琴;郭九峰;王蕴华;朱晓清;聂奇森
单  位:
广西大学轻工与食品工程学院;内蒙古农牧业科学院生物中心;内蒙古农业大学生物工程学院
关键词:
杏鲍菇;多糖;苯酚-硫酸法
摘  要:
本实验研究了苯酚-硫酸法测定杏鲍菇多糖含量时各因素对结果的影响,并对这些因素进行了优化。通过实验发现,波长为485nm、苯酚用量1.4ml、反应温度40℃、反应时间15min,在10.00~70.00μg/ml范围内吸光度与被测物含量呈良好的线性关系,相关系数r为0.9996,平均回收率99.2%,相对标准偏差(RSD)为1.16%。
译  名:
Study on Phenol-Sulfuric Acid Method for Determination of Polysaccharide Content in Pleurotus eryngii
作  者:
GUO Jin-long1,CHEN You-jun1,SUN Guo-qin2,,GUO Jiu-feng2,WANG Yun-hua2,ZHU Xiao-qing2,NIE Qi-sen3(1.College of Biological Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.Biotechnology Research Center,Inner Mongolia Academy of Agricultural Sciences,Hohhot 010031,China; 3.Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China)
关键词:
Peurotus eryngii;polysaccharide;phenol-sulfuric acid method
摘  要:
The factors affacting the determination of polysaccharide content in Pleurotus eryngii with phenol-sulfuric acid method were optimized. Results showed that under the conditions of detection wavelength 485 nm,phenol content 1.4 ml,reaction temperature 40 ℃ and reaction time 15 min,there is good linearity range from 10.00 to 70.00μg/ml(r=0.9996). The average recovery is 99.2% and the RSD is 1.16%.

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