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当前位置: 首页 > 文章 > HPLC检测鸡组织中脱氢乙酸残留量的研究 中国家禽 2012,34 (24) 25-28
Position: Home > Articles > Residues Detection of Dehydroacetic Acid in Chicken Tissues by High Performance Liquid Chromatography China Poultry 2012,34 (24) 25-28

HPLC检测鸡组织中脱氢乙酸残留量的研究

作  者：
杜玉锋;桂淦;束婧婷;张雨梅
单  位：
中国药科大学药学院;合肥畜牧水产局;扬州大学兽医学院;江苏省家禽科学研究所
关键词：
脱氢乙酸;药物残留;HPLC;鸡组织
摘  要：
为建立鸡组织(肌肉、肝脏、肾脏)中脱氢乙酸(DHA)残留的高效液相色谱(HPLC)检测方法,将鸡组织样品经乙腈提取,正己烷液-液萃取,PAX小柱固相萃取净化后,用乙腈溶解残渣,过滤,HPLC分析。色谱条件为:甲醇-0.02%乙酸铵缓冲溶液(20/80,v/v)为流动相,290nm紫外检测。DHA标准工作液在0.2~5.0mg/L浓度范围内线性关系良好(R2≥0.9996),方法检测限为0.1mg/kg,定量限为0.2mg/kg。各组织中DHA添加浓度为0.5、1.0、5.0mg/kg时,各组织样品中DHA回收率为68.28%~93.49%,日内变异系数在3.38%~7.94%,日间变异系数在4.31%~7.80%。所建立的HPLC方法能够满足动物性组织中DHA的残留检测,可为DHA在动物性食品中的残留消除、最高残留限量及休药期研究提供技术支持。
译  名：
Residues Detection of Dehydroacetic Acid in Chicken Tissues by High Performance Liquid Chromatography
作  者：
DU Yufeng1,GUI Gan2,SHU Jingting3,ZHANG Yumei4(1.College of Pharmacy,China Pharmaceutical University,Nanjing,Jiangsu 210009;2.Animal Husbandry and Fishery Bureau of Hefei City,Hefei,Anhui 238000;3.Jiangsu Institute of Poultry Science,Yangzhou,Jiangsu 225125;4.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009)
关键词：
dehydroacetic acid;high performance liquid chromatography;drug residue;chicken tissue
摘  要：
A high performance liquid chromatography(HPLC)method was established to determine the residues of dehydroacetic acid(DHA)in chicken tissues.The tissue samples(muscle,liver and kidney)were extracted with acetonitrile,liquid-liquid extraction by hexane,purified by PAX solid phase extraction column.A C18 column with ultraviolet absorption detection at 290 nm,using methanol-water which includes 0.02% ammonium acetate(20/80,v/v)as eluent was used in HPLC.A linear calibration curve with coefficient relation(R2≥0.9996)in the range of 0.2 to 5.0 mg/L of DHA concentration was made.When DHA was added to tissues at dose of 0.5,1.0 and 5.0 mg/kg,the average recoveries in different tissues were ranged from 68.28% to 93.49%,with CV% ranged from 3.38% to 7.94% within-day and 4.31% to 7.80% between-day.The limit of detection and limit of quantitation of DHA were observed at 0.1 mg/kg and 0.2 mg/kg respectively.The established method was suitable for the determination of DHA residues in animal foods,be also useful to the investigation of DHA depletion,the maximum residue limit and its withdraw time.

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