单 位：
河北联合大学基础医学院药理教研室;广西壮族自治区食品药品检验所中华全国供销合作总社济南果品研究院河北联合大学基础医学院药理教研室;广西壮族自治区食品药品检验所;中华全国供销合作总社济南果品研究院
关键词：
β受体激动剂;前处理;体内代谢;表面增强拉曼光谱法
摘 要：
探索用表面增强拉曼光谱法检测盐酸克伦特罗等β受体激动剂于给药和停药期间在猪尿液、毛发、粪便和组织中的浓度,为快速检出在饲养和市场流通环节中的瘦肉精提供方法支持。在生猪饲料中以10mg/kg的量添加一种β受体激动剂,并连续喂养14d,然后停止给药,使用正常饲料。在给药期间于l、2、3、4、6、8、10和14d分别采集尿液、毛发和粪便,通过前处理后与表面信号增强剂等体积混合,测定其中的β受体激动剂浓度。停药后每天剪去少量毛发,前处理后测定其中的主药的蓄积浓度。结果显示,样品回收率在94.0%以上,相对标准偏差小于2%。盐酸克伦特罗、硫酸沙丁胺醇、硫酸特布他林、班布特罗、盐酸氯丙那林、富马酸福莫特罗的标准溶液在0.05~50μg/L质量浓度范围呈良好线性关系,定量下限为0.05μg/L;尿液、粪便、毛发样品中在1~50μg/L质量浓度范围呈良好线性关系,定量下限为1μg/L。盐酸莱克多巴胺的标准溶液在0.1~50μg/L质量浓度范围呈良好线性关系,定量下限为0.1μg/L。结果表明,该研究测试方法便捷灵敏,用于β受体激动剂在猪体内代谢分析,以及检测其在猪各组织部位残留情况尤为适宜。
译 名：
Catabolism and residue of β agonists in swine by surface-enhanced Raman scattering spectrometry
作 者：
GAN Sheng;LAI Qing-niao;LI Zhi-cheng;HAN Ting;WU Chao-quan;Guangxi Institute for Food and Drug Control;Institute of Light Industry and Food Engineering,Guangxi University;Preclinical College,Hebei United University;
关键词：
β agonists;;pre-processing;;catabolism in-vivo;;surface-enhanced Raman scattering spectrometry
摘 要：
To track the catabolic route and clearance ofβagonists,e.g.clenbuterol hydrochloride,in vivo by surface-enhanced Raman scattering spectrometry,and provide a method of fast screeningβagonists used in animal feeds and in market circulation.βagonists were administered in the swine forage for 14 days.The urine,hair and feces at 1st,2nd,3rd,4th,6th,8th,10 th and 14 th was collected,pre-processed,blended with an equal volume of surface-enhancer,and the concentrations ofβagonists were tested.At the withdrawal period,the clearance of API in hair was calculated and analyzed.The sample recovery was above 94.0%,with the RSD within 2%.The reference standards of clenbuterol hydrochloride,salbutamol sulfate,terbutaline surfate,bambuterol,clorprenaline hydrochloride,and formoterol fumarate were in good linear at the concentration of 0.05-50μg/L,with the LoQ at 0.05μg/L;the samples of urine,feces and hair were in good linear at 1-50μg/L,with the LoQ at 1μg/L;the reference standard of ractopamine hydrochloride was in good linear at 0.1-50μg/L,with the LoQ at 0.1μg/L.The method was proved to be fast and sensitive,applycable to the catabolic analysis ofβagonists in swine and the residue detection of API in swine hair.
