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Position: Home > Articles > Preparation of Silica Gel Immunoaffinity Column for Chloramphenicol Determination FOOD SCIENCE 2012,33 (24) 352-355

硅胶载体氯霉素免疫亲和柱的制备

作  者:
张灿;周婷;陆介宇;段玉清;王硕;于娇
单  位:
天津科技大学食品工程与生物技术学院;江苏大学食品与生物工程学院
关键词:
免疫亲和柱;硅胶;氯霉素抗体;戊二醛法
摘  要:
以硅胶为载体,通过硅烷偶联剂3-氨基丙基三乙氧基硅烷在其表面引入氨基,采用戊二醛法与氯霉素抗体进行偶联。该方法制备的免疫亲和柱对氯霉素抗体的偶联率达到72%,对氯霉素的最大吸附容量达到80ng/mL柱体积,选择含70%甲醇的PBS为洗脱溶液。以牛奶为检测对象分别采用3种不同方法进行样品处理,利用免疫亲和柱实现对样品中痕量残留的氯霉素纯化富集并进行ELISA检测。
译  名:
Preparation of Silica Gel Immunoaffinity Column for Chloramphenicol Determination
作  者:
ZHANG Can1,2,ZHOU Ting1,LU Jie-yu1,DUAN Yu-qing1,WANG Shuo2,YU Jiao2(1.School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;2.College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)
关键词:
immunoaffinity column;silica gel;anti-chloramphenicol antibody;glutaraldehyde
摘  要:
Silica gel was used as a carrier for preparing an immunoaffinity column(IAC) to determine chloramphenicol.Amino groups were introduced to the surface of silica gel using 3-aminopropyltriethoxysilane(APTES) as a silane coupling agent and the modified silica gel was conjugated with anti-chloramphenicol antibodies using glutaraldehyde.As a result,a conjugation ratio of 72% was obtained.The maximum adsorption capacity of the prepared IAC was 80 ng/mL.The elution solvent was PBS containing 70% methanol.After sample pretreatment by three different methods,chloramphenicol residues in milk were purified and enriched using the IAC and determined by ELISA

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